A protocol for measuring cue reactivity in a rat model of cocaine use disorder

Andrea L. Dimet, Irma Cisneros, Robert G. Fox, Sonja J. Stutz, Noelle Anastasio, Kathryn Cunningham, Kelly Dineley

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cocaine use disorder (CUD) follows a trajectory of repetitive self-administration during which previously neutral stimuli gain incentive value. Cue reactivity, the sensitivity to cues previously linked with the drug-taking experience, plays a prominent role in human craving during abstinence. Cue reactivity can be assessed as the attentional orientation toward drug-associated cues that is measurable as appetitive approach behavior in both preclinical and human studies. Herein describes an assessment of cue reactivity in rats trained to self-administer cocaine. Cocaine self-administration is paired with the presentation of discrete cues that act as conditioned reinforcers (i.e., house light, stimulus light, infusion pump sounds). Following a period of abstinence, lever presses in the cocaine self-administration context accompanied by the discrete cues previously paired with cocaine infusion are measured as cue reactivity. This model is useful to explore neurobiological mechanisms underlying cue reactivity processes as well as to assess pharmacotherapies to suppress cue reactivity and therefore, modify relapse vulnerability. Advantages of the model include its translational relevance, and its face and predictive validities. The primary limitation of the model is that the cue reactivity task can only be performed infrequently and must only be used in short duration (e.g., 1 hour), otherwise rats will begin to extinguish the pairing of the discrete cues with the cocaine stimulus. The model is extendable to any positively reinforcing stimulus paired with discrete cues; though particularly applicable to drugs of abuse, this model may hold future applications in fields such as obesity, where palatable food rewards can act as positively reinforcing stimuli.

Original languageEnglish (US)
Article numbere55864
JournalJournal of Visualized Experiments
Volume2018
Issue number136
DOIs
StatePublished - Jun 20 2018
Externally publishedYes

Fingerprint

Cocaine
Cues
Rats
Self Administration
Drug therapy
Street Drugs
Pharmaceutical Preparations
Trajectories
Acoustic waves
Pumps
Appetitive Behavior
Choice Behavior
Light
Infusion Pumps
Reward
Reproducibility of Results
Motivation
Obesity
Recurrence
Drug Therapy

Keywords

  • Abstinence
  • Cocaine
  • Cue
  • Cue reactivity
  • Drug
  • Drug-seeking
  • Issue 136
  • Neuroscience
  • Operant conditioning
  • Rats
  • Relapse
  • Self-administration
  • Substance use disorder
  • Translational

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

A protocol for measuring cue reactivity in a rat model of cocaine use disorder. / Dimet, Andrea L.; Cisneros, Irma; Fox, Robert G.; Stutz, Sonja J.; Anastasio, Noelle; Cunningham, Kathryn; Dineley, Kelly.

In: Journal of Visualized Experiments, Vol. 2018, No. 136, e55864, 20.06.2018.

Research output: Contribution to journalArticle

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