A rapid and sensitive method for detecting human cells in xenogeneic hosts

Edward A. Zompa, Mary Pat Moyer, Lisa D. Cain, Jane E. Bottenstein, Claire E. Hulsebosch

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    2 Scopus citations

    Abstract

    Xenografts, specifically transplantation of human cells into other species, are a valuable tool in preclinical transplantation experiments. A central issue is accurate identification of the grafted cells, particularly in cases in which cellular migration has occurred. We report that detection of grafted human cells can be achieved by in situ hybridization techniques using human centromeric probes which result in unambiguous nuclear labeling. The resulting reaction can be combined with immunocytochemical or histochemical techniques for cell-type characterization.

    Original languageEnglish (US)
    Pages (from-to)928-931
    Number of pages4
    JournalTransplantation
    Volume66
    Issue number7
    DOIs
    StatePublished - Oct 15 1998

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    ASJC Scopus subject areas

    • Transplantation

    Cite this

    Zompa, E. A., Moyer, M. P., Cain, L. D., Bottenstein, J. E., & Hulsebosch, C. E. (1998). A rapid and sensitive method for detecting human cells in xenogeneic hosts. Transplantation, 66(7), 928-931. https://doi.org/10.1097/00007890-199810150-00020