A rapid method for the inactivation of virus infectivity prior to assay for interferons

A. D.T. Barrett; N. Hunt; N. J. Dimmock

doi:10.1016/0166-0934(84)90072-7

A rapid method for the inactivation of virus infectivity prior to assay for interferons

A. D.T. Barrett
, N. Hunt
, N. J. Dimmock

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Virus infectivity in samples of culture medium or suspensions of animal tissue which are required for interferon assay can be rapidly and conveniently inactivated by overnight incubation with beta-propiolactone (BPL). As BPL hydrolyses spontaneously samples can be assayed with no further treatment. BPL does not affect the interfering activity of a, β or γ mouse interferons.

Original language	English (US)
Pages (from-to)	349-351
Number of pages	3
Journal	Journal of Virological Methods
Volume	8
Issue number	4
DOIs	https://doi.org/10.1016/0166-0934(84)90072-7
State	Published - Jul 1984
Externally published	Yes

Keywords

assay of interferons α, β, γ
beta-propiolactone
inactivation of virus

ASJC Scopus subject areas

Virology

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10.1016/0166-0934(84)90072-7

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@article{cf5a9b1834f04c6bb89e875149d00723,

title = "A rapid method for the inactivation of virus infectivity prior to assay for interferons",

abstract = "Virus infectivity in samples of culture medium or suspensions of animal tissue which are required for interferon assay can be rapidly and conveniently inactivated by overnight incubation with beta-propiolactone (BPL). As BPL hydrolyses spontaneously samples can be assayed with no further treatment. BPL does not affect the interfering activity of a, β or γ mouse interferons.",

keywords = "assay of interferons α, β, γ, beta-propiolactone, inactivation of virus",

author = "Barrett, \{A. D.T.\} and N. Hunt and Dimmock, \{N. J.\}",

note = "Funding Information: We thank Andy Carver for suggesting the use of beta-propiolactone and Chris Sutton for providing y-interferon preparations. A.D.T.B. was funded by the Science and Engineering Research Council and N.H. by the Medical Research Council.",

year = "1984",

month = jul,

doi = "10.1016/0166-0934(84)90072-7",

language = "English (US)",

volume = "8",

pages = "349--351",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier B.V.",

number = "4",

}

TY - JOUR

T1 - A rapid method for the inactivation of virus infectivity prior to assay for interferons

AU - Barrett, A. D.T.

AU - Hunt, N.

AU - Dimmock, N. J.

N1 - Funding Information: We thank Andy Carver for suggesting the use of beta-propiolactone and Chris Sutton for providing y-interferon preparations. A.D.T.B. was funded by the Science and Engineering Research Council and N.H. by the Medical Research Council.

PY - 1984/7

Y1 - 1984/7

N2 - Virus infectivity in samples of culture medium or suspensions of animal tissue which are required for interferon assay can be rapidly and conveniently inactivated by overnight incubation with beta-propiolactone (BPL). As BPL hydrolyses spontaneously samples can be assayed with no further treatment. BPL does not affect the interfering activity of a, β or γ mouse interferons.

AB - Virus infectivity in samples of culture medium or suspensions of animal tissue which are required for interferon assay can be rapidly and conveniently inactivated by overnight incubation with beta-propiolactone (BPL). As BPL hydrolyses spontaneously samples can be assayed with no further treatment. BPL does not affect the interfering activity of a, β or γ mouse interferons.

KW - assay of interferons α, β, γ

KW - beta-propiolactone

KW - inactivation of virus

UR - https://www.scopus.com/pages/publications/0021154073

UR - https://www.scopus.com/pages/publications/0021154073#tab=citedBy

U2 - 10.1016/0166-0934(84)90072-7

DO - 10.1016/0166-0934(84)90072-7

M3 - Article

C2 - 6206084

AN - SCOPUS:0021154073

SN - 0166-0934

VL - 8

SP - 349

EP - 351

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 4

ER -

A rapid method for the inactivation of virus infectivity prior to assay for interferons

Abstract

Keywords

ASJC Scopus subject areas

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