TY - JOUR
T1 - A real-time reverse transcriptase polymerase chain reaction for detection and quantification of vesiculovirus
AU - Tolardo, Aline Lavado
AU - de Souza, William Marciel
AU - Romeiro, Marilia Farignoli
AU - Vieira, Luiz Carlos
AU - Luna, Luciano Kleber de Souza
AU - Henriques, Dyana Alves
AU - de Araujo, Jansen
AU - Siqueira, Carlos Eduardo Hassegawa
AU - Colombo, Tatiana Elias
AU - Aquino, Victor Hugo
AU - da Fonseca, Benedito Antonio Lopes
AU - Bronzoni, Roberta Vieira de Morais
AU - Nogueira, Maurício Lacerda
AU - Durigon, Edison Luiz
AU - Figueiredo, Luiz Tadeu Moraes
N1 - Publisher Copyright:
© 2016, Fundacao Oswaldo Cruz. All rights reserved.
PY - 2016/6
Y1 - 2016/6
N2 - Vesiculoviruses (VSV) are zoonotic viruses that cause vesicular stomatitis disease in cattle, horses and pigs, as well as sporadic human cases of acute febrile illness. Therefore, diagnosis of VSV infections by reliable laboratory techniques is important to allow a proper case management and implementation of strategies for the containment of virus spread. We show here a sensitive and reproducible real-time reverse transcriptase polymerase chain reaction (RT-PCR) for detection and quantification of VSV. The assay was evaluated with arthropods and serum samples obtained from horses, cattle and patients with acute febrile disease. The real-time RT-PCR amplified the Piry, Carajas, Alagoas and Indiana Vesiculovirus at a melting temperature 81.02 ± 0.8°C, and the sensitivity of assay was estimated in 10 RNA copies/mL to the Piry Vesiculovirus. The viral genome has been detected in samples of horses and cattle, but not detected in human sera or arthropods. Thus, this assay allows a preliminary differential diagnosis of VSV infections.
AB - Vesiculoviruses (VSV) are zoonotic viruses that cause vesicular stomatitis disease in cattle, horses and pigs, as well as sporadic human cases of acute febrile illness. Therefore, diagnosis of VSV infections by reliable laboratory techniques is important to allow a proper case management and implementation of strategies for the containment of virus spread. We show here a sensitive and reproducible real-time reverse transcriptase polymerase chain reaction (RT-PCR) for detection and quantification of VSV. The assay was evaluated with arthropods and serum samples obtained from horses, cattle and patients with acute febrile disease. The real-time RT-PCR amplified the Piry, Carajas, Alagoas and Indiana Vesiculovirus at a melting temperature 81.02 ± 0.8°C, and the sensitivity of assay was estimated in 10 RNA copies/mL to the Piry Vesiculovirus. The viral genome has been detected in samples of horses and cattle, but not detected in human sera or arthropods. Thus, this assay allows a preliminary differential diagnosis of VSV infections.
KW - Diagnosis of vesicular stomatitis
KW - Quantitative real-time RT-PCR
KW - Vesiculovirus
KW - Zoonotic virus
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U2 - 10.1590/0074-02760150456
DO - 10.1590/0074-02760150456
M3 - Article
C2 - 27276185
AN - SCOPUS:84971467958
SN - 0074-0276
VL - 111
SP - 385
EP - 390
JO - Memorias do Instituto Oswaldo Cruz
JF - Memorias do Instituto Oswaldo Cruz
IS - 6
ER -