A recombinant envelope protein-based enzyme-linked immunosorbent assay for West Nile virus serodiagnosis.

Tian Wang, Louis A. Magnarelli, John F. Anderson, L. Hannah Gould, Sandra L. Bushmich, Susan J. Wong, Erol Fikrig

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Recombinant West Nile virus envelope (E) protein was examined in enzyme-linked immunosorbent assay (ELISA) to detect antibodies elicited during West Nile virus infection. Horses (nine of 10) and humans (six of six) with confirmed West Nile virus infection had IgG and/or IgM antibodies to the E protein. Antibodies to the recombinant West Nile virus membrane and nonstructural 1 proteins were not detected in any of these sera. An E protein-based ELISA may aid in the serological diagnosis of West Nile virus infection.

Original languageEnglish (US)
Pages (from-to)105-109
Number of pages5
JournalVector Borne Zoonotic Dis
Volume2
Issue number2
StatePublished - Jun 2002
Externally publishedYes

Fingerprint

West Nile virus
Serologic Tests
Recombinant Proteins
Virus Diseases
Enzyme-Linked Immunosorbent Assay
Antibodies
Viral Envelope Proteins
Horses
Immunoglobulin M
Proteins
Immunoglobulin G
Membranes
Serum

ASJC Scopus subject areas

  • Infectious Diseases
  • Parasitology

Cite this

Wang, T., Magnarelli, L. A., Anderson, J. F., Gould, L. H., Bushmich, S. L., Wong, S. J., & Fikrig, E. (2002). A recombinant envelope protein-based enzyme-linked immunosorbent assay for West Nile virus serodiagnosis. Vector Borne Zoonotic Dis, 2(2), 105-109.

A recombinant envelope protein-based enzyme-linked immunosorbent assay for West Nile virus serodiagnosis. / Wang, Tian; Magnarelli, Louis A.; Anderson, John F.; Gould, L. Hannah; Bushmich, Sandra L.; Wong, Susan J.; Fikrig, Erol.

In: Vector Borne Zoonotic Dis, Vol. 2, No. 2, 06.2002, p. 105-109.

Research output: Contribution to journalArticle

Wang, T, Magnarelli, LA, Anderson, JF, Gould, LH, Bushmich, SL, Wong, SJ & Fikrig, E 2002, 'A recombinant envelope protein-based enzyme-linked immunosorbent assay for West Nile virus serodiagnosis.', Vector Borne Zoonotic Dis, vol. 2, no. 2, pp. 105-109.
Wang T, Magnarelli LA, Anderson JF, Gould LH, Bushmich SL, Wong SJ et al. A recombinant envelope protein-based enzyme-linked immunosorbent assay for West Nile virus serodiagnosis. Vector Borne Zoonotic Dis. 2002 Jun;2(2):105-109.
Wang, Tian ; Magnarelli, Louis A. ; Anderson, John F. ; Gould, L. Hannah ; Bushmich, Sandra L. ; Wong, Susan J. ; Fikrig, Erol. / A recombinant envelope protein-based enzyme-linked immunosorbent assay for West Nile virus serodiagnosis. In: Vector Borne Zoonotic Dis. 2002 ; Vol. 2, No. 2. pp. 105-109.
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