@article{9668a9c80c194c22ae5ac3543805316b,
title = "A recombinant VSV-vectored vaccine rapidly protects nonhuman primates against heterologous lethal Lassa fever",
abstract = "Lassa virus (LASV) is recognized by the World Health Organization as one of the top five pathogens likely to cause a severe outbreak. A recent unprecedented resurgence of LASV in Nigeria caused by genetically diverse strains underscores the need for licensed medical countermeasures. Single-injection vaccines that can rapidly control outbreaks and confer long-term immunity are needed. Vaccination of cynomolgus monkeys with a recombinant vesicular stomatitis virus vector expressing the glycoprotein precursor of LASV lineage IV strain Josiah (rVSVΔG-LASV-GPC) induces fast-acting protection in monkeys challenged 3 or 7 days later with a genetically heterologous lineage II isolate of LASV from Nigeria, while nonspecifically vaccinated control animals succumb to challenge. The rVSVΔG-LASV-GPC vaccine induces rapid activation of adaptive immunity and the transcription of natural killer (NK) cell-affiliated mRNAs. This study demonstrates that rVSVΔG-LASV-GPC may provide rapid protection in humans against LASV infections in cases where immediate public-health intervention is required.",
keywords = "CP: Immunology, Lassa virus, T cell, arenavirus, hemorrhagic fever, immunity, natural killer cell, primate, vaccine, vesicular stomatitis virus",
author = "Cross, {Robert W.} and Courtney Woolsey and Abhishek Prasad and Viktoriya Borisevich and Agans, {Krystle N.} and Deer, {Daniel J.} and Geisbert, {Joan B.} and Dobias, {Natalie S.} and Karla Fenton and Geisbert, {Thomas W.}",
note = "Funding Information: The authors would like to thank the UTMB Animal Resource Center for husbandry support of laboratory animals and Kevin Melody for assistance with the animal studies. Opinions, interpretations, conclusions, and recommendations are those of the authors and are not necessarily endorsed by the University of Texas Medical Branch. This study was supported by the US Army Medical Research Acquisition Activity contract number W81XWH1910027 to T.W.G. and Department of Health and Human Services , National Institutes of Health grant number UC7AI094660 for BSL-4 operations support of the Galveston National Laboratory. This study was supported in part by and Department of Health and Human Services , National Institutes of Health U01AI151801 . Funding Information: The authors would like to thank the UTMB Animal Resource Center for husbandry support of laboratory animals and Kevin Melody for assistance with the animal studies. Opinions, interpretations, conclusions, and recommendations are those of the authors and are not necessarily endorsed by the University of Texas Medical Branch. This study was supported by the US Army Medical Research Acquisition Activity contract number W81XWH1910027 to T.W.G. and Department of Health and Human Services, National Institutes of Health grant number UC7AI094660 for BSL-4 operations support of the Galveston National Laboratory. This study was supported in part by and Department of Health and Human Services, National Institutes of Health U01AI151801. R.W.C. and T.W.G. conceived and designed the animal challenge experiments. R.W.C. D.J.D. J.B.G. and T.W.G. performed the animal procedures. R.W.C. A.N.P. C.W. D.J.D. and T.W.G. conducted clinical observations. K.N.A. and V.B. performed the clinical pathology. K.N.A. performed the PCR assays. V.B. performed the LASV infectivity assays and plaque reduction neutralization tests. C.W. performed the Nanostring assays, ELISAs, and ELISPOTs. N.S.D. performed the IHC assays. K.A.F. performed gross pathologic, histologic, and immunohistochemical analysis of the data. All authors analyzed the data. C.W. A.N.P. K.A.F. and T.W.G. wrote the paper. R.W.C. edited the paper. All authors had access to all of the data and approved the final version of the manuscript. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = jul,
day = "19",
doi = "10.1016/j.celrep.2022.111094",
language = "English (US)",
volume = "40",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "3",
}