A resourceful giant: APMV is able to interfere with the human type I interferon system

Lorena C.F. Silva, Gabriel M.F. Almeida, Danilo B. Oliveira, Fábio P. Dornas, Rafael Kroon Campos, Bernard La Scola, Paulo C.P. Ferreira, Erna G. Kroon, Jônatas S. Abrahão

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Acanthamoeba polyphaga mimivirus (APMV) is a giant, double-stranded virus of the Mimiviridae family that was discovered in 2003. Recent studies have shown that this virus is able to replicate in murine and human phagocytes and might be considered a putative human pathogen that causes pneumonia. However, there is little data regarding APMV and its host defense relationship. In the present study, we investigated how some components of the interferon (IFN) system are stimulated by APMV in human peripheral blood mononuclear cells (PBMCs) and how APMV replication is affected by IFN treatment. Our results demonstrated that APMV is able to replicate in human PBMCs, inducing type I Interferons (IFNs) but inhibiting interferon stimulated genes (ISG) induction by viroceptor and STAT-1 and STAT-2 dephosphorylation independent mechanisms. We also showed that APMV is resistant to the antiviral action of interferon-alpha2 (IFNA2) but is sensitive to the antiviral action of interferon-beta (IFNB1). Our results demonstrated the productive infection of professional phagocytes with APMV and showed that this virus is recognized by the immune system of vertebrates and inhibits it. It provides the first data regarding APMV and the IFN system interaction and raise new and relevant evolutional questions about the relationship between APMV and vertebrate hosts.

Original languageEnglish (US)
Pages (from-to)187-195
Number of pages9
JournalMicrobes and Infection
Volume16
Issue number3
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Keywords

  • Interferon
  • Mimiviridae
  • PBMCs
  • Phagocytes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases

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    Silva, L. C. F., Almeida, G. M. F., Oliveira, D. B., Dornas, F. P., Kroon Campos, R., La Scola, B., Ferreira, P. C. P., Kroon, E. G., & Abrahão, J. S. (2014). A resourceful giant: APMV is able to interfere with the human type I interferon system. Microbes and Infection, 16(3), 187-195. https://doi.org/10.1016/j.micinf.2013.11.011