A retrospective analysis of sera collected by the Hemorrhagic Fever Commission during the Korean Conflict

James LeDuc, Thomas Ksiazek, Cynthia A. Rossi, Joel M. Dalrymple

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

More than 600 sera from 245 patients with a clinical diagnosis of hemorrhagic fever were preserved by the Hemorrhagic Fever Commission during the Korean Conflict, 1951-1954. These sera were tested for IgM- and IgG-specific antibodies to Hantaan virus by enzyme immunoassay and for hantaviral antigen by immunoassay; one serum from each patient was tested by plaque reduction neutralization using both Hantaan and Seoul viruses. Only 15 patients failed to develop antihantaviral antibodies; most sera contained high titered IgM antibody on admission, and all were IgM-seropositive by day 7 after onset. Attempts to detect hantaviral antigen were unsuccessful. All seropositive patients had highest plaque reduction neutralization titers to Hantaan virus, suggesting that this virus was responsible for the disease seen. These results confirm that hemorrhagic fever of the Korean Conflict was due to Hantaan virus and demonstrate that measurement of specific IgM antibody is the method of choice for diagnosis of acute disease.

Original languageEnglish (US)
Pages (from-to)1182-1184
Number of pages3
JournalJournal of Infectious Diseases
Volume162
Issue number5
StatePublished - Nov 1990
Externally publishedYes

Fingerprint

Korean War
Hantaan virus
Immunoglobulin M
Fever
Antibodies
Serum
Seoul virus
Antigens
Acute Disease
Immunoenzyme Techniques
Immunoassay
Immunoglobulin G
Viruses

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

A retrospective analysis of sera collected by the Hemorrhagic Fever Commission during the Korean Conflict. / LeDuc, James; Ksiazek, Thomas; Rossi, Cynthia A.; Dalrymple, Joel M.

In: Journal of Infectious Diseases, Vol. 162, No. 5, 11.1990, p. 1182-1184.

Research output: Contribution to journalArticle

@article{5a824f1d27af40cca3cccb9742fc11d6,
title = "A retrospective analysis of sera collected by the Hemorrhagic Fever Commission during the Korean Conflict",
abstract = "More than 600 sera from 245 patients with a clinical diagnosis of hemorrhagic fever were preserved by the Hemorrhagic Fever Commission during the Korean Conflict, 1951-1954. These sera were tested for IgM- and IgG-specific antibodies to Hantaan virus by enzyme immunoassay and for hantaviral antigen by immunoassay; one serum from each patient was tested by plaque reduction neutralization using both Hantaan and Seoul viruses. Only 15 patients failed to develop antihantaviral antibodies; most sera contained high titered IgM antibody on admission, and all were IgM-seropositive by day 7 after onset. Attempts to detect hantaviral antigen were unsuccessful. All seropositive patients had highest plaque reduction neutralization titers to Hantaan virus, suggesting that this virus was responsible for the disease seen. These results confirm that hemorrhagic fever of the Korean Conflict was due to Hantaan virus and demonstrate that measurement of specific IgM antibody is the method of choice for diagnosis of acute disease.",
author = "James LeDuc and Thomas Ksiazek and Rossi, {Cynthia A.} and Dalrymple, {Joel M.}",
year = "1990",
month = "11",
language = "English (US)",
volume = "162",
pages = "1182--1184",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "5",

}

TY - JOUR

T1 - A retrospective analysis of sera collected by the Hemorrhagic Fever Commission during the Korean Conflict

AU - LeDuc, James

AU - Ksiazek, Thomas

AU - Rossi, Cynthia A.

AU - Dalrymple, Joel M.

PY - 1990/11

Y1 - 1990/11

N2 - More than 600 sera from 245 patients with a clinical diagnosis of hemorrhagic fever were preserved by the Hemorrhagic Fever Commission during the Korean Conflict, 1951-1954. These sera were tested for IgM- and IgG-specific antibodies to Hantaan virus by enzyme immunoassay and for hantaviral antigen by immunoassay; one serum from each patient was tested by plaque reduction neutralization using both Hantaan and Seoul viruses. Only 15 patients failed to develop antihantaviral antibodies; most sera contained high titered IgM antibody on admission, and all were IgM-seropositive by day 7 after onset. Attempts to detect hantaviral antigen were unsuccessful. All seropositive patients had highest plaque reduction neutralization titers to Hantaan virus, suggesting that this virus was responsible for the disease seen. These results confirm that hemorrhagic fever of the Korean Conflict was due to Hantaan virus and demonstrate that measurement of specific IgM antibody is the method of choice for diagnosis of acute disease.

AB - More than 600 sera from 245 patients with a clinical diagnosis of hemorrhagic fever were preserved by the Hemorrhagic Fever Commission during the Korean Conflict, 1951-1954. These sera were tested for IgM- and IgG-specific antibodies to Hantaan virus by enzyme immunoassay and for hantaviral antigen by immunoassay; one serum from each patient was tested by plaque reduction neutralization using both Hantaan and Seoul viruses. Only 15 patients failed to develop antihantaviral antibodies; most sera contained high titered IgM antibody on admission, and all were IgM-seropositive by day 7 after onset. Attempts to detect hantaviral antigen were unsuccessful. All seropositive patients had highest plaque reduction neutralization titers to Hantaan virus, suggesting that this virus was responsible for the disease seen. These results confirm that hemorrhagic fever of the Korean Conflict was due to Hantaan virus and demonstrate that measurement of specific IgM antibody is the method of choice for diagnosis of acute disease.

UR - http://www.scopus.com/inward/record.url?scp=0025172218&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025172218&partnerID=8YFLogxK

M3 - Article

C2 - 1977805

AN - SCOPUS:0025172218

VL - 162

SP - 1182

EP - 1184

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 5

ER -