A rhamnose-deficient lipopolysaccharide mutant of Rhizobium sp. IRBG74 is defective in root colonization and beneficial interactions with its flooding-tolerant hosts Sesbania cannabina and wetland rice

Shubhajit Mitra, Arijit Mukherjee, Audrey Wiley-Kalil, Seema Das, Heather Owen, Pallavolu M. Reddy, Jean Michel Ané, Euan K. James, Prasad Gyaneshwar

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Rhizobium sp. IRBG74 develops a classical nitrogen-fixing symbiosis with the aquatic legume Sesbania cannabina (Retz.). It also promotes the growth of wetland rice (Oryza sativa L.), but little is known about the rhizobial determinants important for these interactions. In this study, we analyzed the colonization of S. cannabina and rice using a strain of Rhizobium sp. IRBG74 dually marked with β-glucuronidase and the green fluorescent protein. This bacterium colonized S. cannabina by crack entry and through root hair infection under flooded and non-flooded conditions, respectively. Rhizobium sp. IRBG74 colonized the surfaces of wetland rice roots, but also entered them at the base of lateral roots. It became endophytically established within intercellular spaces in the rice cortex, and intracellularly within epidermal and hypodermal cells. A mutant of Rhizobium sp. IRBG74 altered in the synthesis of the rhamnose-containing O-antigen exhibited significant defects, not only in nodulation and symbiotic nitrogen fixation with S. cannabina, but also in rice colonization and plant growth promotion. Supplementation with purified lipopolysaccharides from the wild-type strain, but not from the mutant, restored the beneficial colonization of rice roots, but not fully effective nodulation of S. cannabina. Commonalities and differences in the rhizobial colonization of the roots of wetland legume and rice hosts are discussed.

Original languageEnglish (US)
Pages (from-to)5869-5884
Number of pages16
JournalJournal of Experimental Botany
Volume67
Issue number19
DOIs
StatePublished - Oct 1 2016
Externally publishedYes

Fingerprint

Sesbania cannabina
Sesbania
Rhamnose
Rhizobium
Wetlands
rhamnose
lipopolysaccharides
Lipopolysaccharides
wetlands
rice
mutants
nodulation
Fabaceae
legumes
growth promotion
intercellular spaces
O Antigens
Nitrogen Fixation
root hairs
Symbiosis

Keywords

  • GFP
  • GUS
  • lateral root base (LRB) entry
  • lipopolysaccharide (LPS)
  • nitrogen fixation
  • plant growth-promoting rhizobacteria (PGPRs)

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

A rhamnose-deficient lipopolysaccharide mutant of Rhizobium sp. IRBG74 is defective in root colonization and beneficial interactions with its flooding-tolerant hosts Sesbania cannabina and wetland rice. / Mitra, Shubhajit; Mukherjee, Arijit; Wiley-Kalil, Audrey; Das, Seema; Owen, Heather; Reddy, Pallavolu M.; Ané, Jean Michel; James, Euan K.; Gyaneshwar, Prasad.

In: Journal of Experimental Botany, Vol. 67, No. 19, 01.10.2016, p. 5869-5884.

Research output: Contribution to journalArticle

Mitra, Shubhajit ; Mukherjee, Arijit ; Wiley-Kalil, Audrey ; Das, Seema ; Owen, Heather ; Reddy, Pallavolu M. ; Ané, Jean Michel ; James, Euan K. ; Gyaneshwar, Prasad. / A rhamnose-deficient lipopolysaccharide mutant of Rhizobium sp. IRBG74 is defective in root colonization and beneficial interactions with its flooding-tolerant hosts Sesbania cannabina and wetland rice. In: Journal of Experimental Botany. 2016 ; Vol. 67, No. 19. pp. 5869-5884.
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AU - Mukherjee, Arijit

AU - Wiley-Kalil, Audrey

AU - Das, Seema

AU - Owen, Heather

AU - Reddy, Pallavolu M.

AU - Ané, Jean Michel

AU - James, Euan K.

AU - Gyaneshwar, Prasad

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AB - Rhizobium sp. IRBG74 develops a classical nitrogen-fixing symbiosis with the aquatic legume Sesbania cannabina (Retz.). It also promotes the growth of wetland rice (Oryza sativa L.), but little is known about the rhizobial determinants important for these interactions. In this study, we analyzed the colonization of S. cannabina and rice using a strain of Rhizobium sp. IRBG74 dually marked with β-glucuronidase and the green fluorescent protein. This bacterium colonized S. cannabina by crack entry and through root hair infection under flooded and non-flooded conditions, respectively. Rhizobium sp. IRBG74 colonized the surfaces of wetland rice roots, but also entered them at the base of lateral roots. It became endophytically established within intercellular spaces in the rice cortex, and intracellularly within epidermal and hypodermal cells. A mutant of Rhizobium sp. IRBG74 altered in the synthesis of the rhamnose-containing O-antigen exhibited significant defects, not only in nodulation and symbiotic nitrogen fixation with S. cannabina, but also in rice colonization and plant growth promotion. Supplementation with purified lipopolysaccharides from the wild-type strain, but not from the mutant, restored the beneficial colonization of rice roots, but not fully effective nodulation of S. cannabina. Commonalities and differences in the rhizobial colonization of the roots of wetland legume and rice hosts are discussed.

KW - GFP

KW - GUS

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KW - nitrogen fixation

KW - plant growth-promoting rhizobacteria (PGPRs)

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