A role for a rat homolog of staufen in the transport of RNA to neuronal dendrites

Shao-Jun Tang, Dan Meulemans, Luis Vazquez, Nalini Colaco, Erin Schuman

Research output: Contribution to journalArticle

170 Citations (Scopus)

Abstract

RNAs are present in dendrites and may be used for local protein synthesis in response to synaptic activity. To begin to understand dendritic RNA targeting, we cloned a rat homolog of staufen, a Drosophila gene that participates in mRNA targeting during development. In hippocampal neurons, rat staufen protein displays a microtubule-dependent somatodendritic distribution pattern that overlaps with dendritic RNAs. To determine whether r-staufen is required for dendritic RNA targeting, we constructed a mutant version containing the RNA binding domains (stau-RBD) but lacking the C-terminal portion potentially involved in dendritic targeting. Stau-RBD expression was restricted to the cell bodies and proximal dendrites. Expression of stau-RBD significantly decreased, while overexpression of wild-type r-staufen increased, the amount of dendritic mRNA. Taken together, these results suggest that the rat staufen protein plays an important role in the delivery of RNA to dendrites.

Original languageEnglish (US)
Pages (from-to)463-475
Number of pages13
JournalNeuron
Volume32
Issue number3
DOIs
StatePublished - Nov 8 2001
Externally publishedYes

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RNA Transport
Dendrites
RNA
Messenger RNA
Microtubules
Drosophila
Neurons
Genes
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

A role for a rat homolog of staufen in the transport of RNA to neuronal dendrites. / Tang, Shao-Jun; Meulemans, Dan; Vazquez, Luis; Colaco, Nalini; Schuman, Erin.

In: Neuron, Vol. 32, No. 3, 08.11.2001, p. 463-475.

Research output: Contribution to journalArticle

Tang, Shao-Jun ; Meulemans, Dan ; Vazquez, Luis ; Colaco, Nalini ; Schuman, Erin. / A role for a rat homolog of staufen in the transport of RNA to neuronal dendrites. In: Neuron. 2001 ; Vol. 32, No. 3. pp. 463-475.
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