A role for stem cell factor and C-kit in the murine intestinal tract secretory response to cholera toxin

Gary R. Klimpel, Ashok Chopra, Keith E. Langley, Jette Wypych, Charles A. Annable, Dominique Kaiserlian, Peter B. Ernst, Johnny Peterson

Research output: Contribution to journalArticle

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Abstract

The role of stem cell factor (SCF) and its receptor (c-kit) in the intestinal secretory response to cholera toxin (CT) was investigated using a ligated intestinal loop model in mice having mutations in the dominant white spotting (W) locus and the steel (Sl) locus. W/W(v) mice, which express an aberrant form of the c-kit protein, failed to give an intestinal secretory response after luminal CT challenge. In contrast, W/W(v) mice and their control littermates had equivalent intestinal secretory responses to Escherichia coli heat-stable enterotoxin (STa). Sl/Sl(d) mice, which express only a soluble truncated form of SCF, also gave a significantly reduced intestinal secretory response to CT when compared to the secretory response of their littermate controls. The unresponsiveness of W/W(v) mice to CT was restricted to the intestinal tract since these mice had foot pad swelling responses to CT challenge that were equivalent to their littermate controls. Restoration of mast cells in W/W(v) mice by bone marrow transplantation of control littermate bone marrow did not reverse the CT-unresponsiveness of the intestinal tract. Histological evaluation of the gastrointestinal tract from W/W(v) mice showed a normal distribution of enterochromaffin cells (ECC). CT challenges of either ligand intestinal loops from C57BL/6 mice or a mouse intestinal epithelial cell line (MODE-K) resulted in elevated levels of mRNA for SCF. MODE-K cells exposed to CT also had enhanced expression of c-kit. Finally, fluid obtained from CT-challenged ligated intestinal loops from C57BL/6 mice contained significant levels of SCF. Taken together, the above results suggest that CT-induced intestinal secretory responses are dependent upon SCF-c- kit interactions. These interactions appear to be induced as a consequence of CT stimulation of the intestinal tract and may also play a role in the development or functionality of the enteric nervous system.

Original languageEnglish (US)
Pages (from-to)1931-1942
Number of pages12
JournalJournal of Experimental Medicine
Volume182
Issue number6
DOIs
StatePublished - Dec 1 1995

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Stem Cell Factor
Cholera Toxin
Steel
Proto-Oncogene Proteins c-kit
Inbred C57BL Mouse
Enterochromaffin Cells
Enteric Nervous System
Metrorrhagia
Enterotoxins
Normal Distribution
Bone Marrow Transplantation
Mast Cells
Gastrointestinal Tract
Foot
Hot Temperature
Bone Marrow
Epithelial Cells
Escherichia coli
Ligands

ASJC Scopus subject areas

  • Immunology

Cite this

A role for stem cell factor and C-kit in the murine intestinal tract secretory response to cholera toxin. / Klimpel, Gary R.; Chopra, Ashok; Langley, Keith E.; Wypych, Jette; Annable, Charles A.; Kaiserlian, Dominique; Ernst, Peter B.; Peterson, Johnny.

In: Journal of Experimental Medicine, Vol. 182, No. 6, 01.12.1995, p. 1931-1942.

Research output: Contribution to journalArticle

Klimpel, Gary R. ; Chopra, Ashok ; Langley, Keith E. ; Wypych, Jette ; Annable, Charles A. ; Kaiserlian, Dominique ; Ernst, Peter B. ; Peterson, Johnny. / A role for stem cell factor and C-kit in the murine intestinal tract secretory response to cholera toxin. In: Journal of Experimental Medicine. 1995 ; Vol. 182, No. 6. pp. 1931-1942.
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