A role for synaptotagmin (p65) in regulated exocytosis

Lisa Elferink, Michael R. Peterson, Richard H. Scheller

Research output: Contribution to journalArticle

204 Citations (Scopus)

Abstract

Proteins that are specifically localized to synaptic vesicles in the nervous system have been proposed to mediate aspects of synaptic transmission. Antibodies raised against the cytoplasmic domains of five of these proteins, vamp, rab3A, synaptophysin, synaptotagmin, and SV2, were used to investigate their function. Microinjection of monoclonal and polyclonal antibodies raised against synaptotagmin (p65), but not the other vesicle proteins, decreases K+/Ca2+-mediated dopamine β-hydroxylase surface staining, a measure of regulated secretion in PC12 cells. Microinjection of a soluble fragment of synaptotagmin encompassing one of the domains homologous to the C2 regulatory region of protein kinase C, but lacking the membrane anchor, also inhibits evoked dopamine β-hydroxylase surface staining. These results provide support for the hypothesis that synaptotagmin, a Ca2+- and phospholipid-binding protein, is important for regulated exocytosis in neurons.

Original languageEnglish (US)
Pages (from-to)153-159
Number of pages7
JournalCell
Volume72
Issue number1
DOIs
StatePublished - Jan 15 1993
Externally publishedYes

Fingerprint

Synaptotagmins
Exocytosis
Microinjections
Mixed Function Oxygenases
rab3A GTP-Binding Protein
Dopamine
Staining and Labeling
Synaptophysin
Proteins
Antibodies
Synaptic Vesicles
Nucleic Acid Regulatory Sequences
PC12 Cells
Neurology
Anchors
Synaptic Transmission
Protein Kinase C
Nervous System
Neurons
Phospholipids

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

A role for synaptotagmin (p65) in regulated exocytosis. / Elferink, Lisa; Peterson, Michael R.; Scheller, Richard H.

In: Cell, Vol. 72, No. 1, 15.01.1993, p. 153-159.

Research output: Contribution to journalArticle

Elferink, Lisa ; Peterson, Michael R. ; Scheller, Richard H. / A role for synaptotagmin (p65) in regulated exocytosis. In: Cell. 1993 ; Vol. 72, No. 1. pp. 153-159.
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