A Role for the Mre11-Rad50-Xrs2 Complex in Gene Expression and Chromosome Organization

Romain Forey, Antoine Barthe, Mireille Tittel-Elmer, Maxime Wery, Marie Bénédicte Barrault, Cécile Ducrot, Andrew Seeber, Nils Krietenstein, Ugo Szachnowski, Magdalena Skrzypczak, Krzysztof Ginalski, Maga Rowicka, Jennifer A. Cobb, Oliver J. Rando, Julie Soutourina, Michel Werner, Karine Dubrana, Susan M. Gasser, Antonin Morillon, Philippe PaseroArmelle Lengronne, Jérôme Poli

Research output: Contribution to journalArticlepeer-review

Abstract

Mre11-Rad50-Xrs2 (MRX) is a highly conserved complex with key roles in various aspects of DNA repair. Here, we report a new function for MRX in limiting transcription in budding yeast. We show that MRX interacts physically and colocalizes on chromatin with the transcriptional co-regulator Mediator. MRX restricts transcription of coding and noncoding DNA by a mechanism that does not require the nuclease activity of Mre11. MRX is required to tether transcriptionally active loci to the nuclear pore complex (NPC), and it also promotes large-scale gene-NPC interactions. Moreover, MRX-mediated chromatin anchoring to the NPC contributes to chromosome folding and helps to control gene expression. Together, these findings indicate that MRX has a role in transcription and chromosome organization that is distinct from its known function in DNA repair.

Original languageEnglish (US)
Pages (from-to)183-197.e6
JournalMolecular cell
Volume81
Issue number1
DOIs
StatePublished - Jan 7 2021

Keywords

  • chromosomal interaction domains
  • chromosome folding
  • chromosome organization
  • coding and non-coding transcription control
  • Mediator
  • MRX/N
  • nuclear pore
  • SMC complexes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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