A selective and sensitive method for quantification of endogenous polysulfide production in biological samples

Sofia Iris Bibli, Bert Luck, Sven Zukunft, Janina Wittig, Wei Chen, Ming Xian, Andreas Papapetropoulos, Jiong Hu, Ingrid Fleming

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Hydrogen sulfide (H2S) is a gasotransmitter that regulates cellular homeostasis and impacts on multiple physiological and pathophysiological processes. However, it exerts many of its biological actions indirectly via the formation of H2S-derived sulfane sulfur species/polysulfides. Because of the high reactivity of sulfur species, the detection of H2S-derived polysulfides in biological systems is challenging and currently used methods are neither sensitive nor quantitative. Herein, we describe a LC-MS/MS-based method that makes use of Sulfane Sulfur Probe 4 to detect endogenously generated polysulfides in biological samples in a selective, sensitive and quantitative manner. The results indicate a large variability in the activity of the H2S-generating enzymes in different murine organs, but the method described was able to detect intracellular levels of polysulfides in the nanomolar range and identify cystathionine γ-lyase as the major intracellular source of sulfane sulfur species/polysulfides in murine endothelial cells and hearts. The protocol described can be applied to a variety of biological samples for the quantification of the H2S-derived polysulfides and has the potential to increase understanding on the control and consequences of this gaseous transmitter.

Original languageEnglish (US)
Pages (from-to)295-304
Number of pages10
JournalRedox Biology
Volume18
DOIs
StatePublished - Sep 1 2018
Externally publishedYes

Fingerprint

Sulfur
Gasotransmitters
Cystathionine
Physiological Phenomena
Hydrogen Sulfide
Lyases
Endothelial cells
Biological systems
polysulfide
Transmitters
Homeostasis
Endothelial Cells
Enzymes

Keywords

  • Biological samples
  • LC-MS/MS
  • Polysulfide quantification
  • SSP4

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

Cite this

A selective and sensitive method for quantification of endogenous polysulfide production in biological samples. / Bibli, Sofia Iris; Luck, Bert; Zukunft, Sven; Wittig, Janina; Chen, Wei; Xian, Ming; Papapetropoulos, Andreas; Hu, Jiong; Fleming, Ingrid.

In: Redox Biology, Vol. 18, 01.09.2018, p. 295-304.

Research output: Contribution to journalArticle

Bibli, Sofia Iris ; Luck, Bert ; Zukunft, Sven ; Wittig, Janina ; Chen, Wei ; Xian, Ming ; Papapetropoulos, Andreas ; Hu, Jiong ; Fleming, Ingrid. / A selective and sensitive method for quantification of endogenous polysulfide production in biological samples. In: Redox Biology. 2018 ; Vol. 18. pp. 295-304.
@article{a85554f21d1d4009ae1aad681b1faf28,
title = "A selective and sensitive method for quantification of endogenous polysulfide production in biological samples",
abstract = "Hydrogen sulfide (H2S) is a gasotransmitter that regulates cellular homeostasis and impacts on multiple physiological and pathophysiological processes. However, it exerts many of its biological actions indirectly via the formation of H2S-derived sulfane sulfur species/polysulfides. Because of the high reactivity of sulfur species, the detection of H2S-derived polysulfides in biological systems is challenging and currently used methods are neither sensitive nor quantitative. Herein, we describe a LC-MS/MS-based method that makes use of Sulfane Sulfur Probe 4 to detect endogenously generated polysulfides in biological samples in a selective, sensitive and quantitative manner. The results indicate a large variability in the activity of the H2S-generating enzymes in different murine organs, but the method described was able to detect intracellular levels of polysulfides in the nanomolar range and identify cystathionine γ-lyase as the major intracellular source of sulfane sulfur species/polysulfides in murine endothelial cells and hearts. The protocol described can be applied to a variety of biological samples for the quantification of the H2S-derived polysulfides and has the potential to increase understanding on the control and consequences of this gaseous transmitter.",
keywords = "Biological samples, LC-MS/MS, Polysulfide quantification, SSP4",
author = "Bibli, {Sofia Iris} and Bert Luck and Sven Zukunft and Janina Wittig and Wei Chen and Ming Xian and Andreas Papapetropoulos and Jiong Hu and Ingrid Fleming",
year = "2018",
month = "9",
day = "1",
doi = "10.1016/j.redox.2018.07.016",
language = "English (US)",
volume = "18",
pages = "295--304",
journal = "Redox Biology",
issn = "2213-2317",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - A selective and sensitive method for quantification of endogenous polysulfide production in biological samples

AU - Bibli, Sofia Iris

AU - Luck, Bert

AU - Zukunft, Sven

AU - Wittig, Janina

AU - Chen, Wei

AU - Xian, Ming

AU - Papapetropoulos, Andreas

AU - Hu, Jiong

AU - Fleming, Ingrid

PY - 2018/9/1

Y1 - 2018/9/1

N2 - Hydrogen sulfide (H2S) is a gasotransmitter that regulates cellular homeostasis and impacts on multiple physiological and pathophysiological processes. However, it exerts many of its biological actions indirectly via the formation of H2S-derived sulfane sulfur species/polysulfides. Because of the high reactivity of sulfur species, the detection of H2S-derived polysulfides in biological systems is challenging and currently used methods are neither sensitive nor quantitative. Herein, we describe a LC-MS/MS-based method that makes use of Sulfane Sulfur Probe 4 to detect endogenously generated polysulfides in biological samples in a selective, sensitive and quantitative manner. The results indicate a large variability in the activity of the H2S-generating enzymes in different murine organs, but the method described was able to detect intracellular levels of polysulfides in the nanomolar range and identify cystathionine γ-lyase as the major intracellular source of sulfane sulfur species/polysulfides in murine endothelial cells and hearts. The protocol described can be applied to a variety of biological samples for the quantification of the H2S-derived polysulfides and has the potential to increase understanding on the control and consequences of this gaseous transmitter.

AB - Hydrogen sulfide (H2S) is a gasotransmitter that regulates cellular homeostasis and impacts on multiple physiological and pathophysiological processes. However, it exerts many of its biological actions indirectly via the formation of H2S-derived sulfane sulfur species/polysulfides. Because of the high reactivity of sulfur species, the detection of H2S-derived polysulfides in biological systems is challenging and currently used methods are neither sensitive nor quantitative. Herein, we describe a LC-MS/MS-based method that makes use of Sulfane Sulfur Probe 4 to detect endogenously generated polysulfides in biological samples in a selective, sensitive and quantitative manner. The results indicate a large variability in the activity of the H2S-generating enzymes in different murine organs, but the method described was able to detect intracellular levels of polysulfides in the nanomolar range and identify cystathionine γ-lyase as the major intracellular source of sulfane sulfur species/polysulfides in murine endothelial cells and hearts. The protocol described can be applied to a variety of biological samples for the quantification of the H2S-derived polysulfides and has the potential to increase understanding on the control and consequences of this gaseous transmitter.

KW - Biological samples

KW - LC-MS/MS

KW - Polysulfide quantification

KW - SSP4

UR - http://www.scopus.com/inward/record.url?scp=85050890373&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85050890373&partnerID=8YFLogxK

U2 - 10.1016/j.redox.2018.07.016

DO - 10.1016/j.redox.2018.07.016

M3 - Article

C2 - 30077923

AN - SCOPUS:85050890373

VL - 18

SP - 295

EP - 304

JO - Redox Biology

JF - Redox Biology

SN - 2213-2317

ER -