A sensitive radioreceptor assay for determining scopolamine concentrations in plasma and urine

Nitza M. Cintrón, Yu‐Ming ‐M Chen

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

A sensitive and reliable procedure for the quantitation of low picogram levels of scopolamine in plasma and urine is described. The method consists of two steps, a preparative extraction step using C18 columns (Sep‐Pak), followed by an analytical quantitation step involving a muscarinic radioreceptor assay. The extraction efficiency of the C18 columns was 85–95% for both plasma and urine over a wide concentration range. When [3H]methyl scopolamine is used as a tracer, the assay can detect picogram concentrations (>25 pg) of scopolamine (base) in plasma and urine. The applicability of the procedure for therapeutic drug monitoring of scopolamine was demonstrated by using the method to determine plasma levels in humans after transdermal administration.

Original languageEnglish (US)
Pages (from-to)328-332
Number of pages5
JournalJournal of Pharmaceutical Sciences
Volume76
Issue number4
DOIs
StatePublished - Apr 1987
Externally publishedYes

ASJC Scopus subject areas

  • Pharmaceutical Science

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