A sequential staining technique for karyotypic analysis of interspecific somatic cell hybrids

Michael J. Keherly, John Papaconstantinou, Jerome L. Mccombs

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Abstract

We describe a sequential staining technique for the karyotypic analysis of interspecific mouse X human somatic cell hybrids. Fluorescence in situ hybridization of samples, previously stained using standard trypsin/ Giemsa protocols, was instrumental in the identification of human chromosomes present in hybrid lines. This procedure not only provided a simple distinction between human and mouse chromosomes, but it also allowed the visualization and monitoring of human sequences present in interspecific translocations and subchromosomal fragments.

Original languageEnglish (US)
Pages (from-to)361-364
Number of pages4
JournalExperimental Cell Research
Volume205
Issue number2
StatePublished - 1993

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ASJC Scopus subject areas

  • Cell Biology

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