A single amino acid change in the E2 glycoprotein of Venezuelan equine encephalitis virus affects replication and dissemination in Aedes aegypti mosquitoes

T. M. Woodward, B. R. Miller, B. J. Beaty, D. W. Trent, J. T. Roehrig

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Four monoclonal antibody-resistant variants (MARVs) of Venezuelan equine encephalitis (VEE) virus were used to study mosquito-virus interactions. In vitro experiments using an Aedes albopictus cell line, C6/36, demonstrated that an amino acid change in the glycoprotein E2(h) epitope (MARV 1A3B-7) decreased virus growth when compared with the wild-type, Trinidad donkey virus, and its vaccine derivative, TC-83. The MARVs replicated as efficiently as the parent virus when inoculated into Aedes aegypti mosquitoes, but MARV 1A3B-7 was restricted in its ability to infect and disseminate from the midgut following oral infection. These results demonstrate that a single amino acid change in the E2 glycoprotein can affect the ability of VEE virus to replicate and disseminate in Ae. aegypti mosquitoes.

Original languageEnglish (US)
Pages (from-to)2431-2435
Number of pages5
JournalJournal of General Virology
Volume72
Issue number10
DOIs
StatePublished - Jan 1 1991

ASJC Scopus subject areas

  • Virology

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