A single amino acid in the M1 protein responsible for the different pathogenic potentials of H5N1 highly pathogenic avian influenza virus strains

Naganori Nao, Masahiro Kajihara, Rashid Manzoor, Junki Maruyama, Reiko Yoshida, Mieko Muramatsu, Hiroko Miyamoto, Manabu Igarashi, Nao Eguchi, Masahiro Sato, Tatsunari Kondoh, Masatoshi Okamatsu, Yoshihiro Sakoda, Hiroshi Kida, Ayato Takada

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Two highly pathogenic avian influenza virus strains, A/duck/Hokkaido/WZ83/2010 (H5N1) (WZ83) and A/duck/Hokkaido/WZ101/2010 (H5N1) (WZ101), which were isolated from wild ducks in Japan, were found to be genetically similar, with only two amino acid differences in their M1 and PB1 proteins at positions 43 and 317, respectively. We found that both WZ83 and WZ101 caused lethal infection in chickens but WZ101 killed them more rapidly than WZ83. Interestingly, ducks experimentally infected with WZ83 showed no or only mild clinical symptoms, whereas WZ101 was highly lethal. We then generated reassortants between these viruses and found that exchange of the M gene segment completely switched the pathogenic phenotype in both chickens and ducks, indicating that the difference in the pathogenicity for these avian species between WZ83 and WZ101 was determined by only a single amino acid in the M1 protein. It was also found that WZ101 showed higher pathogenicity than WZ83 in mice and that WZ83, whose M gene was replaced with that of WZ101, showed higher pathogenicity than wild-type WZ83, although this reassortant virus was not fully pathogenic compared to wild-type WZ101. These results suggest that the amino acid at position 43 of the M1 protein is one of the factors contributing to the pathogenicity of H5N1 highly pathogenic avian influenza viruses in both avian and mammalian hosts.

Original languageEnglish (US)
Article numbere0137989
JournalPLoS One
Volume10
Issue number9
DOIs
StatePublished - Sep 14 2015
Externally publishedYes

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Influenza in Birds
Ducks
Orthomyxoviridae
Viruses
Influenza A virus
ducks
pathogenicity
Virulence
Amino Acids
Reassortant Viruses
amino acids
Japan
Proteins
lethal genes
proteins
Genes
Chickens
chickens
viruses
signs and symptoms (animals and humans)

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

A single amino acid in the M1 protein responsible for the different pathogenic potentials of H5N1 highly pathogenic avian influenza virus strains. / Nao, Naganori; Kajihara, Masahiro; Manzoor, Rashid; Maruyama, Junki; Yoshida, Reiko; Muramatsu, Mieko; Miyamoto, Hiroko; Igarashi, Manabu; Eguchi, Nao; Sato, Masahiro; Kondoh, Tatsunari; Okamatsu, Masatoshi; Sakoda, Yoshihiro; Kida, Hiroshi; Takada, Ayato.

In: PLoS One, Vol. 10, No. 9, e0137989, 14.09.2015.

Research output: Contribution to journalArticle

Nao, N, Kajihara, M, Manzoor, R, Maruyama, J, Yoshida, R, Muramatsu, M, Miyamoto, H, Igarashi, M, Eguchi, N, Sato, M, Kondoh, T, Okamatsu, M, Sakoda, Y, Kida, H & Takada, A 2015, 'A single amino acid in the M1 protein responsible for the different pathogenic potentials of H5N1 highly pathogenic avian influenza virus strains', PLoS One, vol. 10, no. 9, e0137989. https://doi.org/10.1371/journal.pone.0137989
Nao, Naganori ; Kajihara, Masahiro ; Manzoor, Rashid ; Maruyama, Junki ; Yoshida, Reiko ; Muramatsu, Mieko ; Miyamoto, Hiroko ; Igarashi, Manabu ; Eguchi, Nao ; Sato, Masahiro ; Kondoh, Tatsunari ; Okamatsu, Masatoshi ; Sakoda, Yoshihiro ; Kida, Hiroshi ; Takada, Ayato. / A single amino acid in the M1 protein responsible for the different pathogenic potentials of H5N1 highly pathogenic avian influenza virus strains. In: PLoS One. 2015 ; Vol. 10, No. 9.
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AU - Maruyama, Junki

AU - Yoshida, Reiko

AU - Muramatsu, Mieko

AU - Miyamoto, Hiroko

AU - Igarashi, Manabu

AU - Eguchi, Nao

AU - Sato, Masahiro

AU - Kondoh, Tatsunari

AU - Okamatsu, Masatoshi

AU - Sakoda, Yoshihiro

AU - Kida, Hiroshi

AU - Takada, Ayato

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