A single-dose of intranasal vaccination with a live-attenuated SARS-CoV-2 vaccine candidate promotes protective mucosal and systemic immunity

Awadalkareem Adam; Birte Kalveram; John Yun Chung Chen; Jason Yeung; Leslie Rodriguez; Ankita Singh; Pei Yong Shi; Xuping Xie; Tian Wang

doi:10.1038/s41541-023-00753-4

A single-dose of intranasal vaccination with a live-attenuated SARS-CoV-2 vaccine candidate promotes protective mucosal and systemic immunity

Awadalkareem Adam, Birte Kalveram, John Yun Chung Chen, Jason Yeung, Leslie Rodriguez, Ankita Singh, Pei Yong Shi, Xuping Xie, Tian Wang

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

An attenuated SARS-CoV-2 virus with modified viral transcriptional regulatory sequences and deletion of open-reading frames 3, 6, 7 and 8 (∆3678) was previously reported to protect hamsters from SARS-CoV-2 infection and transmission. Here we report that a single-dose intranasal vaccination of ∆3678 protects K18-hACE2 mice from wild-type or variant SARS-CoV-2 challenge. Compared with wild-type virus infection, the ∆3678 vaccination induces equivalent or higher levels of lung and systemic T cell, B cell, IgA, and IgG responses. The results suggest ∆3678 as an attractive mucosal vaccine candidate to boost pulmonary immunity against SARS-CoV-2.

Original language	English (US)
Article number	160
Journal	npj Vaccines
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41541-023-00753-4
State	Published - Dec 2023

ASJC Scopus subject areas

Immunology
Pharmacology
Infectious Diseases
Pharmacology (medical)

Access to Document

10.1038/s41541-023-00753-4

Cite this

@article{9674364192bb42beb1bffe4ab02169f8,

title = "A single-dose of intranasal vaccination with a live-attenuated SARS-CoV-2 vaccine candidate promotes protective mucosal and systemic immunity",

abstract = "An attenuated SARS-CoV-2 virus with modified viral transcriptional regulatory sequences and deletion of open-reading frames 3, 6, 7 and 8 (∆3678) was previously reported to protect hamsters from SARS-CoV-2 infection and transmission. Here we report that a single-dose intranasal vaccination of ∆3678 protects K18-hACE2 mice from wild-type or variant SARS-CoV-2 challenge. Compared with wild-type virus infection, the ∆3678 vaccination induces equivalent or higher levels of lung and systemic T cell, B cell, IgA, and IgG responses. The results suggest ∆3678 as an attractive mucosal vaccine candidate to boost pulmonary immunity against SARS-CoV-2.",

author = "Awadalkareem Adam and Birte Kalveram and Chen, {John Yun Chung} and Jason Yeung and Leslie Rodriguez and Ankita Singh and Shi, {Pei Yong} and Xuping Xie and Tian Wang",

note = "Publisher Copyright: {\textcopyright} 2023, Springer Nature Limited.",

year = "2023",

month = dec,

doi = "10.1038/s41541-023-00753-4",

language = "English (US)",

volume = "8",

journal = "npj Vaccines",

issn = "2059-0105",

publisher = "Nature Publishing Group",

number = "1",

}

TY - JOUR

T1 - A single-dose of intranasal vaccination with a live-attenuated SARS-CoV-2 vaccine candidate promotes protective mucosal and systemic immunity

AU - Adam, Awadalkareem

AU - Kalveram, Birte

AU - Chen, John Yun Chung

AU - Yeung, Jason

AU - Rodriguez, Leslie

AU - Singh, Ankita

AU - Shi, Pei Yong

AU - Xie, Xuping

AU - Wang, Tian

PY - 2023/12

Y1 - 2023/12

N2 - An attenuated SARS-CoV-2 virus with modified viral transcriptional regulatory sequences and deletion of open-reading frames 3, 6, 7 and 8 (∆3678) was previously reported to protect hamsters from SARS-CoV-2 infection and transmission. Here we report that a single-dose intranasal vaccination of ∆3678 protects K18-hACE2 mice from wild-type or variant SARS-CoV-2 challenge. Compared with wild-type virus infection, the ∆3678 vaccination induces equivalent or higher levels of lung and systemic T cell, B cell, IgA, and IgG responses. The results suggest ∆3678 as an attractive mucosal vaccine candidate to boost pulmonary immunity against SARS-CoV-2.

AB - An attenuated SARS-CoV-2 virus with modified viral transcriptional regulatory sequences and deletion of open-reading frames 3, 6, 7 and 8 (∆3678) was previously reported to protect hamsters from SARS-CoV-2 infection and transmission. Here we report that a single-dose intranasal vaccination of ∆3678 protects K18-hACE2 mice from wild-type or variant SARS-CoV-2 challenge. Compared with wild-type virus infection, the ∆3678 vaccination induces equivalent or higher levels of lung and systemic T cell, B cell, IgA, and IgG responses. The results suggest ∆3678 as an attractive mucosal vaccine candidate to boost pulmonary immunity against SARS-CoV-2.

UR - http://www.scopus.com/inward/record.url?scp=85174540398&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85174540398&partnerID=8YFLogxK

U2 - 10.1038/s41541-023-00753-4

DO - 10.1038/s41541-023-00753-4

M3 - Article

C2 - 37863935

AN - SCOPUS:85174540398

SN - 2059-0105

VL - 8

JO - npj Vaccines

JF - npj Vaccines

IS - 1

M1 - 160

ER -

A single-dose of intranasal vaccination with a live-attenuated SARS-CoV-2 vaccine candidate promotes protective mucosal and systemic immunity

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this