@article{cf3d9479977d416db4a54f28a7ac4f67,
title = "A single intranasal dose of human parainfluenza virus type 3-vectored vaccine induces effective antibody and memory T cell response in the lungs and protects hamsters against SARS-CoV-2",
abstract = "Respiratory tract vaccination has an advantage of needle-free delivery and induction of mucosal immune response in the portal of SARS-CoV-2 entry. We utilized human parainfluenza virus type 3 vector to generate constructs expressing the full spike (S) protein of SARS-CoV-2, its S1 subunit, or the receptor-binding domain, and tested them in hamsters as single-dose intranasal vaccines. The construct bearing full-length S induced high titers of neutralizing antibodies specific to S protein domains critical to the protein functions. Robust memory T cell responses in the lungs were also induced, which represent an additional barrier to infection and should be less sensitive than the antibody responses to mutations present in SARS-CoV-2 variants. Following SARS-CoV-2 challenge, animals were protected from the disease and detectable viral replication. Vaccination prevented induction of gene pathways associated with inflammation. These results indicate advantages of respiratory vaccination against COVID-19 and inform the design of mucosal SARS-CoV-2 vaccines.",
author = "Ilinykh, {Philipp A.} and Sivakumar Periasamy and Kai Huang and Kuzmina, {Natalia A.} and Palaniappan Ramanathan and Michelle Meyer and Chad Mire and Kuzmin, {Ivan V.} and Preeti Bharaj and Endsley, {Jessica R.} and Maria Chikina and Sealfon, {Stuart C.} and Widen, {Steven G.} and Endsley, {Mark A.} and Alexander Bukreyev",
note = "Funding Information: We are grateful to Dr. Peter L. Collins (NIAID, NIH) for providing the HPIV3 reverse genetics system. We thank Drs. Natalie Thornburg (CDC), David Wentworth (CDC), Mehul Suthar (Emory University) and Kenneth S. Plante (UTMB) for providing SARS-CoV-2 isolates, Dr. Pei-Yong Shi (UTMB) for providing mNeonGreen SARS-CoV-2, Dr. Matthias J. Schnell (Thomas Jefferson University) for providing S gene in pCR-Blunt II-TOPO vector, Dr. Mohd Alfaraj (UTMB Anatomic Pathology core) for histopathology tissue processing, Shannon Zhang (Full Moon BioSystems, Inc.) for peptide microarray slide scanning, and Jill Thompson (UTMB) for preparation of libraries for RNA deep sequencing. We thank the UTMB Animal Resource Center veterinary staff for the technical support of hamster experiments. Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
month = dec,
doi = "10.1038/s41541-022-00471-3",
language = "English (US)",
volume = "7",
journal = "npj Vaccines",
issn = "2059-0105",
publisher = "Nature Publishing Group",
number = "1",
}