A single-nucleotide polymorphism in the gene encoding osteoprotegerin, an anti-inflammatory protein produced in response to infection with diarrheagenic Escherichia coli, is associated with an increased risk of nonsecretory bacterial diarrhea in North American travelers to Mexico

Jamal A. Mohamed, Herbert L. DuPont, Zhi Dong Jiang, Jose Flores, Lily G. Carlin, Jaime Belkind-Gerson, Francisco G. Martinez-Sandoval, Dongchuan Guo, A. Clinton White, Pablo C. Okhuysen

Research output: Contribution to journalArticle

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Abstract

Background. Osteoprotegerin (OPG), an immunoregulatory member of the TNF receptor superfamily, is expressed in inflamed intestinal mucosa. We investigated whether OPG is produced by intestinal epithelial cells and tested the hypothesis that single-nucleotide polymorphisms (SNPs) in the gene encoding OPG (TNFRSF11B) are associated with traveler's diarrhea (TD) among North American travelers to Mexico. Methods. OPG concentration was measured in the supernatants of T84 cells infected with various diarrheagenic Escherichia coli pathotypes. Genotyping was performed for 4 SNPs in the OPG gene for 968 North American travelers with or without TD. Stool samples from travelers with TD were evaluated for the presence of enteric pathogens. Results. T84 cells produced higher OPG levels in response to infection with various diarrheagenic E. coli pathotypes than with E. coli controls (P < .05). A SNP in the exon 1 region of the OPG gene (OPG +1181G>C) was associated with TD in white travelers who stayed in Mexico for >1 week during the summer (P = .009) and for TD due to nonsecretory pathogens (P = .001). Conclusions. Our study suggests that OPG is secreted by intestinal epithelial cells in response to enteropathogens and that a polymorphism in the OPG gene is associated with an increased susceptibility to TD.

Original languageEnglish (US)
Pages (from-to)477-485
Number of pages9
JournalJournal of Infectious Diseases
Volume199
Issue number4
DOIs
StatePublished - Feb 15 2009

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Osteoprotegerin
Mexico
Single Nucleotide Polymorphism
Diarrhea
Anti-Inflammatory Agents
Escherichia coli
Infection
Genes
Proteins
Epithelial Cells
Tumor Necrosis Factor Receptors
Intestinal Mucosa

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

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A single-nucleotide polymorphism in the gene encoding osteoprotegerin, an anti-inflammatory protein produced in response to infection with diarrheagenic Escherichia coli, is associated with an increased risk of nonsecretory bacterial diarrhea in North American travelers to Mexico. / Mohamed, Jamal A.; DuPont, Herbert L.; Jiang, Zhi Dong; Flores, Jose; Carlin, Lily G.; Belkind-Gerson, Jaime; Martinez-Sandoval, Francisco G.; Guo, Dongchuan; White, A. Clinton; Okhuysen, Pablo C.

In: Journal of Infectious Diseases, Vol. 199, No. 4, 15.02.2009, p. 477-485.

Research output: Contribution to journalArticle

Mohamed, Jamal A. ; DuPont, Herbert L. ; Jiang, Zhi Dong ; Flores, Jose ; Carlin, Lily G. ; Belkind-Gerson, Jaime ; Martinez-Sandoval, Francisco G. ; Guo, Dongchuan ; White, A. Clinton ; Okhuysen, Pablo C. / A single-nucleotide polymorphism in the gene encoding osteoprotegerin, an anti-inflammatory protein produced in response to infection with diarrheagenic Escherichia coli, is associated with an increased risk of nonsecretory bacterial diarrhea in North American travelers to Mexico. In: Journal of Infectious Diseases. 2009 ; Vol. 199, No. 4. pp. 477-485.
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abstract = "Background. Osteoprotegerin (OPG), an immunoregulatory member of the TNF receptor superfamily, is expressed in inflamed intestinal mucosa. We investigated whether OPG is produced by intestinal epithelial cells and tested the hypothesis that single-nucleotide polymorphisms (SNPs) in the gene encoding OPG (TNFRSF11B) are associated with traveler's diarrhea (TD) among North American travelers to Mexico. Methods. OPG concentration was measured in the supernatants of T84 cells infected with various diarrheagenic Escherichia coli pathotypes. Genotyping was performed for 4 SNPs in the OPG gene for 968 North American travelers with or without TD. Stool samples from travelers with TD were evaluated for the presence of enteric pathogens. Results. T84 cells produced higher OPG levels in response to infection with various diarrheagenic E. coli pathotypes than with E. coli controls (P < .05). A SNP in the exon 1 region of the OPG gene (OPG +1181G>C) was associated with TD in white travelers who stayed in Mexico for >1 week during the summer (P = .009) and for TD due to nonsecretory pathogens (P = .001). Conclusions. Our study suggests that OPG is secreted by intestinal epithelial cells in response to enteropathogens and that a polymorphism in the OPG gene is associated with an increased susceptibility to TD.",
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AU - Mohamed, Jamal A.

AU - DuPont, Herbert L.

AU - Jiang, Zhi Dong

AU - Flores, Jose

AU - Carlin, Lily G.

AU - Belkind-Gerson, Jaime

AU - Martinez-Sandoval, Francisco G.

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AU - White, A. Clinton

AU - Okhuysen, Pablo C.

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