A soluble divalent class I MHC/IgG1 fusion protein activates CD8+ T cells in vivo

Brenna Carey, Monica DeLay, Jane E. Strasser, Claudia Chalk, Kristen Dudley-McClain, Gregg N. Milligan, Hermine I. Brunner, Sherry Thornton, Raphael Hirsch

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

CD8+ T lymphocytes recognize tumor and viral antigens bound to class I major histocompatibility complexes (MHC). Tumors and viruses may evade detection by preventing antigen presentation. The present study was designed to determine whether a soluble divalent fusion protein, containing the extracellular domains of a class I MHC molecule fused to β2-microglobulin and the constant domains of IgG1, could induce an immune response in vivo. Administration to mice of the fusion protein loaded with a tumor peptide induced peptide-specific T cell activation and retarded tumor growth. Administration of the fusion protein loaded with a glycoprotein B (gB) peptide derived from herpes simplex virus type 1 (HSV-1) induced gB-specific cytotoxic T lymphocytes and protected mice from a lethal HSV-1 challenge. These data suggest that antigen-loaded MHC/IgG fusion proteins may enhance T cell immunity in conditions where antigen presentation is altered.

Original languageEnglish (US)
Pages (from-to)65-76
Number of pages12
JournalClinical Immunology
Volume116
Issue number1
DOIs
StatePublished - Jul 1 2005

Keywords

  • CTL
  • MHC
  • T lymphocytes
  • Tumor immunity
  • Vaccination
  • Viral immunity

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Carey, B., DeLay, M., Strasser, J. E., Chalk, C., Dudley-McClain, K., Milligan, G. N., Brunner, H. I., Thornton, S., & Hirsch, R. (2005). A soluble divalent class I MHC/IgG1 fusion protein activates CD8+ T cells in vivo. Clinical Immunology, 116(1), 65-76. https://doi.org/10.1016/j.clim.2005.02.013