A spectrally constrained dual-band normalization technique for protoporphyrin IX quantification in fluorescence-guided surgery

P. A. Valdés, F. Leblond, A. Kim, B. C. Wilson, K. D. Paulsen, D. W. Roberts

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

We report a dual-band normalization technique for in vivo quantification of the metabolic biomarker, protoporphyrin IX (PpIX), during brain tumor resection procedures. The accuracy of the approach was optimized in tissue simulating phantoms with varying absorption and scattering properties, validated with fluorimetric assessments on ex vivo brain tissue, and tested on human data acquired in vivo during fluorescence-guided surgery of brain tumors. The results demonstrate that the dual-band normalization technique allows PpIX concentrations to be accurately quantified by correction with reflectance data recorded and integrated within only two narrow wavelength intervals. The simplicity of the method lends itself to the enticing prospect that the method could be applicable to wide-field applications in quantitative fluorescence imaging and dosimetry in photodynamic therapy.

Original languageEnglish (US)
Pages (from-to)1817-1819
Number of pages3
JournalOptics Letters
Volume37
Issue number11
DOIs
StatePublished - Jun 1 2012
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Fingerprint

Dive into the research topics of 'A spectrally constrained dual-band normalization technique for protoporphyrin IX quantification in fluorescence-guided surgery'. Together they form a unique fingerprint.

Cite this