A strong association between body fat mass and protein profiles in nipple aspirate fluid of healthy premenopausal non-lactating women

Yafei Huang, Manubai Nagamani, Karl Anderson, Alexander Kurosky, Anthony M. Haag, James J. Grady, Leejane Lu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Fluid can be aspirated from the nipples of most non-lactating women. This nipple aspirate fluid (NAF) is a potential source for the discovery of new breast cancer biomarkers. NAF has two distinct protein profiles. Type I NAF is similar to the fluid associated with cystic disease of the breast, whereas type II NAF is enriched in milk-associated proteins. The prevalence of these two profiles differs in healthy women and in breast cancer patients. This study investigated the relationship of these two NAF profiles to reproductive history, body composition, diet, and levels of lipids, steroids and thyroid hormones in healthy premenopausal women (age 30-40 years) who had regular menstrual cycles and normal mammograms and were not taking contraceptive medications. On average, women with the type I NAF profile were older, had more years since last childbirth, were less likely to have breastfed their babies and had higher dietary saturated fat intake, body mass index, body fat mass, and levels of plasma low density lipoproteins than women with the type II profile (P <0.05). Using multiple logistic regression, type I NAF was predicted independently (P <0.05) by higher body fat mass [Odds Ratio (OR) = 3.0; 95% Confidence Interval (CI): 1.5-6.1], more years since last childbirth (OR = 2.6; 95% CI: 1.3-5.2) and a higher percentage of calories from saturated fat (OR = 4.1; 95% CI: 1.1-14.6). These results suggest that protein profiles of NAF might be influenced by amounts or types of dietary and body fat, but further study of the relationship of the two profiles to breast cancer risk is needed.

Original languageEnglish (US)
Pages (from-to)57-66
Number of pages10
JournalBreast Cancer Research and Treatment
Volume104
Issue number1
DOIs
StatePublished - Jul 2007

Fingerprint

Nipple Aspirate Fluid
Adipose Tissue
Proteins
Dietary Fats
Odds Ratio
Confidence Intervals
Breast Neoplasms
Parturition
Fibrocystic Breast Disease
Reproductive History
Nipples
Milk Proteins
Tumor Biomarkers
Menstrual Cycle
Contraceptive Agents
Body Composition
Thyroid Hormones
LDL Lipoproteins
Body Mass Index
Logistic Models

Keywords

  • Breast cancer biomarker
  • Gross cystic disease fluid proteins
  • Milk proteins
  • Obesity
  • Proteome

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

A strong association between body fat mass and protein profiles in nipple aspirate fluid of healthy premenopausal non-lactating women. / Huang, Yafei; Nagamani, Manubai; Anderson, Karl; Kurosky, Alexander; Haag, Anthony M.; Grady, James J.; Lu, Leejane.

In: Breast Cancer Research and Treatment, Vol. 104, No. 1, 07.2007, p. 57-66.

Research output: Contribution to journalArticle

Huang, Yafei ; Nagamani, Manubai ; Anderson, Karl ; Kurosky, Alexander ; Haag, Anthony M. ; Grady, James J. ; Lu, Leejane. / A strong association between body fat mass and protein profiles in nipple aspirate fluid of healthy premenopausal non-lactating women. In: Breast Cancer Research and Treatment. 2007 ; Vol. 104, No. 1. pp. 57-66.
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