A structural analysis of M protein in coronavirus assembly and morphology

Benjamin W. Neuman; Gabriella Kiss; Andreas H. Kunding; David Bhella; M. Fazil Baksh; Stephen Connelly; Ben Droese; Joseph P. Klaus; Shinji Makino; Stanley G. Sawicki; Stuart G. Siddell; Dimitrios G. Stamou; Ian A. Wilson; Peter Kuhn; Michael J. Buchmeier

doi:10.1016/j.jsb.2010.11.021

A structural analysis of M protein in coronavirus assembly and morphology

Benjamin W. Neuman, Gabriella Kiss, Andreas H. Kunding, David Bhella, M. Fazil Baksh, Stephen Connelly, Ben Droese, Joseph P. Klaus, Shinji Makino, Stanley G. Sawicki, Stuart G. Siddell, Dimitrios G. Stamou, Ian A. Wilson, Peter Kuhn, Michael J. Buchmeier

Microbiology And Immunology

Research output: Contribution to journal › Article › peer-review

405 Scopus citations

Abstract

The M protein of coronavirus plays a central role in virus assembly, turning cellular membranes into workshops where virus and host factors come together to make new virus particles. We investigated how M structure and organization is related to virus shape and size using cryo-electron microscopy, tomography and statistical analysis. We present evidence that suggests M can adopt two conformations and that membrane curvature is regulated by one M conformer. Elongated M protein is associated with rigidity, clusters of spikes and a relatively narrow range of membrane curvature. In contrast, compact M protein is associated with flexibility and low spike density. Analysis of several types of virus-like particles and virions revealed that S protein, N protein and genomic RNA each help to regulate virion size and variation, presumably through interactions with M. These findings provide insight into how M protein functions to promote virus assembly.

Original language	English (US)
Pages (from-to)	11-22
Number of pages	12
Journal	Journal of Structural Biology
Volume	174
Issue number	1
DOIs	https://doi.org/10.1016/j.jsb.2010.11.021
State	Published - Apr 2011

Keywords

Coronavirus
Cryo-electron microscopy
Cryo-electron tomography
Pleomorphic virus structure
Viral matrix protein

ASJC Scopus subject areas

Structural Biology

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10.1016/j.jsb.2010.11.021

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Neuman, B. W., Kiss, G., Kunding, A. H., Bhella, D., Baksh, M. F., Connelly, S., Droese, B., Klaus, J. P., Makino, S., Sawicki, S. G., Siddell, S. G., Stamou, D. G., Wilson, I. A., Kuhn, P., & Buchmeier, M. J. (2011). A structural analysis of M protein in coronavirus assembly and morphology. Journal of Structural Biology, 174(1), 11-22. https://doi.org/10.1016/j.jsb.2010.11.021

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title = "A structural analysis of M protein in coronavirus assembly and morphology",

abstract = "The M protein of coronavirus plays a central role in virus assembly, turning cellular membranes into workshops where virus and host factors come together to make new virus particles. We investigated how M structure and organization is related to virus shape and size using cryo-electron microscopy, tomography and statistical analysis. We present evidence that suggests M can adopt two conformations and that membrane curvature is regulated by one M conformer. Elongated M protein is associated with rigidity, clusters of spikes and a relatively narrow range of membrane curvature. In contrast, compact M protein is associated with flexibility and low spike density. Analysis of several types of virus-like particles and virions revealed that S protein, N protein and genomic RNA each help to regulate virion size and variation, presumably through interactions with M. These findings provide insight into how M protein functions to promote virus assembly.",

keywords = "Coronavirus, Cryo-electron microscopy, Cryo-electron tomography, Pleomorphic virus structure, Viral matrix protein",

author = "Neuman, {Benjamin W.} and Gabriella Kiss and Kunding, {Andreas H.} and David Bhella and Baksh, {M. Fazil} and Stephen Connelly and Ben Droese and Klaus, {Joseph P.} and Shinji Makino and Sawicki, {Stanley G.} and Siddell, {Stuart G.} and Stamou, {Dimitrios G.} and Wilson, {Ian A.} and Peter Kuhn and Buchmeier, {Michael J.}",

note = "Funding Information: This research was funded by the NIH/NIAID contract HHSN-266200400058C , AI-059799 , AI-25913 , AI-29984 and AI-72493 , and by the Danish Research Council . Some of the electron microscopy shown here was conducted at the National Resource for Automated Molecular Microscopy which is supported by the National Institutes of Health though the National Center for Research Resources{\textquoteright} P41 program (RR17573). ",

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doi = "10.1016/j.jsb.2010.11.021",

language = "English (US)",

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AU - Stamou, Dimitrios G.

AU - Wilson, Ian A.

AU - Kuhn, Peter

AU - Buchmeier, Michael J.

N1 - Funding Information: This research was funded by the NIH/NIAID contract HHSN-266200400058C , AI-059799 , AI-25913 , AI-29984 and AI-72493 , and by the Danish Research Council . Some of the electron microscopy shown here was conducted at the National Resource for Automated Molecular Microscopy which is supported by the National Institutes of Health though the National Center for Research Resources’ P41 program (RR17573).

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A structural analysis of M protein in coronavirus assembly and morphology

Abstract

Keywords

ASJC Scopus subject areas

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