Abstract
The M protein of coronavirus plays a central role in virus assembly, turning cellular membranes into workshops where virus and host factors come together to make new virus particles. We investigated how M structure and organization is related to virus shape and size using cryo-electron microscopy, tomography and statistical analysis. We present evidence that suggests M can adopt two conformations and that membrane curvature is regulated by one M conformer. Elongated M protein is associated with rigidity, clusters of spikes and a relatively narrow range of membrane curvature. In contrast, compact M protein is associated with flexibility and low spike density. Analysis of several types of virus-like particles and virions revealed that S protein, N protein and genomic RNA each help to regulate virion size and variation, presumably through interactions with M. These findings provide insight into how M protein functions to promote virus assembly.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 11-22 |
| Number of pages | 12 |
| Journal | Journal of Structural Biology |
| Volume | 174 |
| Issue number | 1 |
| DOIs | |
| State | Published - Apr 2011 |
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Keywords
- Coronavirus
- Cryo-electron microscopy
- Cryo-electron tomography
- Pleomorphic virus structure
- Viral matrix protein
ASJC Scopus subject areas
- Structural Biology
Cite this
A structural analysis of M protein in coronavirus assembly and morphology. / Neuman, Benjamin W.; Kiss, Gabriella; Kunding, Andreas H.; Bhella, David; Baksh, M. Fazil; Connelly, Stephen; Droese, Ben; Klaus, Joseph P.; Makino, Shinji; Sawicki, Stanley G.; Siddell, Stuart G.; Stamou, Dimitrios G.; Wilson, Ian A.; Kuhn, Peter; Buchmeier, Michael J.
In: Journal of Structural Biology, Vol. 174, No. 1, 04.2011, p. 11-22.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A structural analysis of M protein in coronavirus assembly and morphology
AU - Neuman, Benjamin W.
AU - Kiss, Gabriella
AU - Kunding, Andreas H.
AU - Bhella, David
AU - Baksh, M. Fazil
AU - Connelly, Stephen
AU - Droese, Ben
AU - Klaus, Joseph P.
AU - Makino, Shinji
AU - Sawicki, Stanley G.
AU - Siddell, Stuart G.
AU - Stamou, Dimitrios G.
AU - Wilson, Ian A.
AU - Kuhn, Peter
AU - Buchmeier, Michael J.
PY - 2011/4
Y1 - 2011/4
N2 - The M protein of coronavirus plays a central role in virus assembly, turning cellular membranes into workshops where virus and host factors come together to make new virus particles. We investigated how M structure and organization is related to virus shape and size using cryo-electron microscopy, tomography and statistical analysis. We present evidence that suggests M can adopt two conformations and that membrane curvature is regulated by one M conformer. Elongated M protein is associated with rigidity, clusters of spikes and a relatively narrow range of membrane curvature. In contrast, compact M protein is associated with flexibility and low spike density. Analysis of several types of virus-like particles and virions revealed that S protein, N protein and genomic RNA each help to regulate virion size and variation, presumably through interactions with M. These findings provide insight into how M protein functions to promote virus assembly.
AB - The M protein of coronavirus plays a central role in virus assembly, turning cellular membranes into workshops where virus and host factors come together to make new virus particles. We investigated how M structure and organization is related to virus shape and size using cryo-electron microscopy, tomography and statistical analysis. We present evidence that suggests M can adopt two conformations and that membrane curvature is regulated by one M conformer. Elongated M protein is associated with rigidity, clusters of spikes and a relatively narrow range of membrane curvature. In contrast, compact M protein is associated with flexibility and low spike density. Analysis of several types of virus-like particles and virions revealed that S protein, N protein and genomic RNA each help to regulate virion size and variation, presumably through interactions with M. These findings provide insight into how M protein functions to promote virus assembly.
KW - Coronavirus
KW - Cryo-electron microscopy
KW - Cryo-electron tomography
KW - Pleomorphic virus structure
KW - Viral matrix protein
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UR - http://www.scopus.com/inward/citedby.url?scp=79952439334&partnerID=8YFLogxK
U2 - 10.1016/j.jsb.2010.11.021
DO - 10.1016/j.jsb.2010.11.021
M3 - Article
C2 - 21130884
AN - SCOPUS:79952439334
VL - 174
SP - 11
EP - 22
JO - Journal of Structural Biology
JF - Journal of Structural Biology
SN - 1047-8477
IS - 1
ER -