A structural analysis of M protein in coronavirus assembly and morphology

Benjamin W. Neuman, Gabriella Kiss, Andreas H. Kunding, David Bhella, M. Fazil Baksh, Stephen Connelly, Ben Droese, Joseph P. Klaus, Shinji Makino, Stanley G. Sawicki, Stuart G. Siddell, Dimitrios G. Stamou, Ian A. Wilson, Peter Kuhn, Michael J. Buchmeier

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The M protein of coronavirus plays a central role in virus assembly, turning cellular membranes into workshops where virus and host factors come together to make new virus particles. We investigated how M structure and organization is related to virus shape and size using cryo-electron microscopy, tomography and statistical analysis. We present evidence that suggests M can adopt two conformations and that membrane curvature is regulated by one M conformer. Elongated M protein is associated with rigidity, clusters of spikes and a relatively narrow range of membrane curvature. In contrast, compact M protein is associated with flexibility and low spike density. Analysis of several types of virus-like particles and virions revealed that S protein, N protein and genomic RNA each help to regulate virion size and variation, presumably through interactions with M. These findings provide insight into how M protein functions to promote virus assembly.

Original languageEnglish (US)
Pages (from-to)11-22
Number of pages12
JournalJournal of Structural Biology
Volume174
Issue number1
DOIs
StatePublished - Apr 2011

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Virion
Virus Assembly
Membranes
Proteins
Electron Microscope Tomography
Viruses
Cryoelectron Microscopy
Protein S
RNA
Education
Coronavirus M protein

Keywords

  • Coronavirus
  • Cryo-electron microscopy
  • Cryo-electron tomography
  • Pleomorphic virus structure
  • Viral matrix protein

ASJC Scopus subject areas

  • Structural Biology

Cite this

Neuman, B. W., Kiss, G., Kunding, A. H., Bhella, D., Baksh, M. F., Connelly, S., ... Buchmeier, M. J. (2011). A structural analysis of M protein in coronavirus assembly and morphology. Journal of Structural Biology, 174(1), 11-22. https://doi.org/10.1016/j.jsb.2010.11.021

A structural analysis of M protein in coronavirus assembly and morphology. / Neuman, Benjamin W.; Kiss, Gabriella; Kunding, Andreas H.; Bhella, David; Baksh, M. Fazil; Connelly, Stephen; Droese, Ben; Klaus, Joseph P.; Makino, Shinji; Sawicki, Stanley G.; Siddell, Stuart G.; Stamou, Dimitrios G.; Wilson, Ian A.; Kuhn, Peter; Buchmeier, Michael J.

In: Journal of Structural Biology, Vol. 174, No. 1, 04.2011, p. 11-22.

Research output: Contribution to journalArticle

Neuman, BW, Kiss, G, Kunding, AH, Bhella, D, Baksh, MF, Connelly, S, Droese, B, Klaus, JP, Makino, S, Sawicki, SG, Siddell, SG, Stamou, DG, Wilson, IA, Kuhn, P & Buchmeier, MJ 2011, 'A structural analysis of M protein in coronavirus assembly and morphology', Journal of Structural Biology, vol. 174, no. 1, pp. 11-22. https://doi.org/10.1016/j.jsb.2010.11.021
Neuman BW, Kiss G, Kunding AH, Bhella D, Baksh MF, Connelly S et al. A structural analysis of M protein in coronavirus assembly and morphology. Journal of Structural Biology. 2011 Apr;174(1):11-22. https://doi.org/10.1016/j.jsb.2010.11.021
Neuman, Benjamin W. ; Kiss, Gabriella ; Kunding, Andreas H. ; Bhella, David ; Baksh, M. Fazil ; Connelly, Stephen ; Droese, Ben ; Klaus, Joseph P. ; Makino, Shinji ; Sawicki, Stanley G. ; Siddell, Stuart G. ; Stamou, Dimitrios G. ; Wilson, Ian A. ; Kuhn, Peter ; Buchmeier, Michael J. / A structural analysis of M protein in coronavirus assembly and morphology. In: Journal of Structural Biology. 2011 ; Vol. 174, No. 1. pp. 11-22.
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