A structural analysis of M protein in coronavirus assembly and morphology

Benjamin W. Neuman; Gabriella Kiss; Andreas H. Kunding; David Bhella; M. Fazil Baksh; Stephen Connelly; Ben Droese; Joseph P. Klaus; Shinji Makino; Stanley G. Sawicki; Stuart G. Siddell; Dimitrios G. Stamou; Ian A. Wilson; Peter Kuhn; Michael J. Buchmeier

doi:10.1016/j.jsb.2010.11.021

A structural analysis of M protein in coronavirus assembly and morphology

Benjamin W. Neuman, Gabriella Kiss, Andreas H. Kunding, David Bhella, M. Fazil Baksh, Stephen Connelly, Ben Droese, Joseph P. Klaus, Shinji Makino, Stanley G. Sawicki, Stuart G. Siddell, Dimitrios G. Stamou, Ian A. Wilson, Peter Kuhn, Michael J. Buchmeier

Microbiology And Immunology

Research output: Contribution to journal › Article › peer-review

250 Scopus citations

Abstract

The M protein of coronavirus plays a central role in virus assembly, turning cellular membranes into workshops where virus and host factors come together to make new virus particles. We investigated how M structure and organization is related to virus shape and size using cryo-electron microscopy, tomography and statistical analysis. We present evidence that suggests M can adopt two conformations and that membrane curvature is regulated by one M conformer. Elongated M protein is associated with rigidity, clusters of spikes and a relatively narrow range of membrane curvature. In contrast, compact M protein is associated with flexibility and low spike density. Analysis of several types of virus-like particles and virions revealed that S protein, N protein and genomic RNA each help to regulate virion size and variation, presumably through interactions with M. These findings provide insight into how M protein functions to promote virus assembly.

Original language	English (US)
Pages (from-to)	11-22
Number of pages	12
Journal	Journal of Structural Biology
Volume	174
Issue number	1
DOIs	https://doi.org/10.1016/j.jsb.2010.11.021
State	Published - Apr 2011

Keywords

Coronavirus
Cryo-electron microscopy
Cryo-electron tomography
Pleomorphic virus structure
Viral matrix protein

ASJC Scopus subject areas

Structural Biology

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10.1016/j.jsb.2010.11.021

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Neuman, B. W., Kiss, G., Kunding, A. H., Bhella, D., Baksh, M. F., Connelly, S., Droese, B., Klaus, J. P., Makino, S., Sawicki, S. G., Siddell, S. G., Stamou, D. G., Wilson, I. A., Kuhn, P., & Buchmeier, M. J. (2011). A structural analysis of M protein in coronavirus assembly and morphology. Journal of Structural Biology, 174(1), 11-22. https://doi.org/10.1016/j.jsb.2010.11.021

A structural analysis of M protein in coronavirus assembly and morphology. / Neuman, Benjamin W.; Kiss, Gabriella; Kunding, Andreas H.; Bhella, David; Baksh, M. Fazil; Connelly, Stephen; Droese, Ben; Klaus, Joseph P.; Makino, Shinji; Sawicki, Stanley G.; Siddell, Stuart G.; Stamou, Dimitrios G.; Wilson, Ian A.; Kuhn, Peter; Buchmeier, Michael J.

In: Journal of Structural Biology, Vol. 174, No. 1, 04.2011, p. 11-22.

Research output: Contribution to journal › Article › peer-review

Neuman, Benjamin W. ; Kiss, Gabriella ; Kunding, Andreas H. ; Bhella, David ; Baksh, M. Fazil ; Connelly, Stephen ; Droese, Ben ; Klaus, Joseph P. ; Makino, Shinji ; Sawicki, Stanley G. ; Siddell, Stuart G. ; Stamou, Dimitrios G. ; Wilson, Ian A. ; Kuhn, Peter ; Buchmeier, Michael J. / A structural analysis of M protein in coronavirus assembly and morphology. In: Journal of Structural Biology. 2011 ; Vol. 174, No. 1. pp. 11-22.

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abstract = "The M protein of coronavirus plays a central role in virus assembly, turning cellular membranes into workshops where virus and host factors come together to make new virus particles. We investigated how M structure and organization is related to virus shape and size using cryo-electron microscopy, tomography and statistical analysis. We present evidence that suggests M can adopt two conformations and that membrane curvature is regulated by one M conformer. Elongated M protein is associated with rigidity, clusters of spikes and a relatively narrow range of membrane curvature. In contrast, compact M protein is associated with flexibility and low spike density. Analysis of several types of virus-like particles and virions revealed that S protein, N protein and genomic RNA each help to regulate virion size and variation, presumably through interactions with M. These findings provide insight into how M protein functions to promote virus assembly.",

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A structural analysis of M protein in coronavirus assembly and morphology

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Keywords

ASJC Scopus subject areas

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