A structurally distinct human mycoplasma protein that generically blocks antigen-antibody union

Rajesh K. Grover, Xueyong Zhu, Travis Nieusma, Teresa Jones, Isabel Boero, Amanda S. MacLeod, Adam Mark, Sherry Niessen, Helen J. Kim, Leopold Kong, Nacyra Assad-Garcia, Keehwan Kwon, Marta Chesi, Vaughn V. Smider, Daniel R. Salomon, Diane F. Jelinek, Robert A. Kyle, Richard Pyles, John I. Glass, Andrew B. Ward & 2 others Ian A. Wilson, Richard A. Lerner

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We report the discovery of a broadly reactive antibody-binding protein (Protein M) from human mycoplasma. The crystal structure of the ectodomain of transmembrane Protein M differs from other known protein structures, as does its mechanism of antibody binding. Protein M binds with high affinity to all types of human and nonhuman immunoglobulin G, predominantly through attachment to the conserved portions of the variable region of the k and l light chains. Protein M blocks antibody-antigen union, likely because of its large C-terminal domain extending over the antibody-combining site, blocking entry to large antigens. Similar to the other immunoglobulin-binding proteins such as Protein A, Protein M as well as its orthologs in other Mycoplasma species could become invaluable reagents in the antibody field.

Original languageEnglish (US)
Pages (from-to)656-661
Number of pages6
JournalScience
Volume343
Issue number6171
DOIs
StatePublished - 2014

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Antigens
Antibodies
Proteins
Carrier Proteins
Staphylococcal Protein A
Immunoglobulins
Immunoglobulin G
Crystal structure
Binding Sites

ASJC Scopus subject areas

  • General

Cite this

Grover, R. K., Zhu, X., Nieusma, T., Jones, T., Boero, I., MacLeod, A. S., ... Lerner, R. A. (2014). A structurally distinct human mycoplasma protein that generically blocks antigen-antibody union. Science, 343(6171), 656-661. https://doi.org/10.1126/science.1246135

A structurally distinct human mycoplasma protein that generically blocks antigen-antibody union. / Grover, Rajesh K.; Zhu, Xueyong; Nieusma, Travis; Jones, Teresa; Boero, Isabel; MacLeod, Amanda S.; Mark, Adam; Niessen, Sherry; Kim, Helen J.; Kong, Leopold; Assad-Garcia, Nacyra; Kwon, Keehwan; Chesi, Marta; Smider, Vaughn V.; Salomon, Daniel R.; Jelinek, Diane F.; Kyle, Robert A.; Pyles, Richard; Glass, John I.; Ward, Andrew B.; Wilson, Ian A.; Lerner, Richard A.

In: Science, Vol. 343, No. 6171, 2014, p. 656-661.

Research output: Contribution to journalArticle

Grover, RK, Zhu, X, Nieusma, T, Jones, T, Boero, I, MacLeod, AS, Mark, A, Niessen, S, Kim, HJ, Kong, L, Assad-Garcia, N, Kwon, K, Chesi, M, Smider, VV, Salomon, DR, Jelinek, DF, Kyle, RA, Pyles, R, Glass, JI, Ward, AB, Wilson, IA & Lerner, RA 2014, 'A structurally distinct human mycoplasma protein that generically blocks antigen-antibody union', Science, vol. 343, no. 6171, pp. 656-661. https://doi.org/10.1126/science.1246135
Grover, Rajesh K. ; Zhu, Xueyong ; Nieusma, Travis ; Jones, Teresa ; Boero, Isabel ; MacLeod, Amanda S. ; Mark, Adam ; Niessen, Sherry ; Kim, Helen J. ; Kong, Leopold ; Assad-Garcia, Nacyra ; Kwon, Keehwan ; Chesi, Marta ; Smider, Vaughn V. ; Salomon, Daniel R. ; Jelinek, Diane F. ; Kyle, Robert A. ; Pyles, Richard ; Glass, John I. ; Ward, Andrew B. ; Wilson, Ian A. ; Lerner, Richard A. / A structurally distinct human mycoplasma protein that generically blocks antigen-antibody union. In: Science. 2014 ; Vol. 343, No. 6171. pp. 656-661.
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