A study of p53 expression in thermal burns of human skin for determination of wound age

Sarah Tarran, Peter Dziewulski, Tamara Sztynda, Neil E.I. Langlois

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Early expression of p53 protein in thermal burns of guinea pig skin has been reported. This study sought to determine if expression occurred in thermal burns of human skin and if immunohistochemical demonstration of p53 protein could be utilised to distinguish ante-mortem from post-mortem injuries as well as indicating the age of a lesion in the living subject. Biopsy samples were obtained from live patients and post-mortem examinations. Immunohistochemistry was used to demonstrate the presence of p53 protein. Staining was assessed by field counting of epithelial cell nuclei. In live subjects there was a tendency for early (six hour to five day) expression, with peak levels occurring around one to two days. Late samples (13 to 23 days) demonstrated minimal or no expression. In contrast, burn wounds from post-mortem examination demonstrated greater staining for p53 protein in the late (28 to 77 day) samples than in the early ones. It appears that expression of p53 protein may assist in the ageing of ante-mortem, but not post-mortem, burn wounds. This implies that results obtained from live subjects may not be applicable to post-mortem material and that any putative method for determining the age of a wound should be tested in both situations.

Original languageEnglish (US)
Pages (from-to)222-226
Number of pages5
JournalMedicine, Science and the Law
Volume44
Issue number3
DOIs
StatePublished - Jan 1 2004
Externally publishedYes

Fingerprint

Burns
Hot Temperature
Skin
Wounds and Injuries
Guinea
examination
Proteins
Autopsy
Staining and Labeling
Cell Nucleus
Guinea Pigs
Epithelial Cells
Immunohistochemistry
Biopsy

ASJC Scopus subject areas

  • Issues, ethics and legal aspects
  • Health Policy
  • Law

Cite this

A study of p53 expression in thermal burns of human skin for determination of wound age. / Tarran, Sarah; Dziewulski, Peter; Sztynda, Tamara; Langlois, Neil E.I.

In: Medicine, Science and the Law, Vol. 44, No. 3, 01.01.2004, p. 222-226.

Research output: Contribution to journalArticle

Tarran, Sarah ; Dziewulski, Peter ; Sztynda, Tamara ; Langlois, Neil E.I. / A study of p53 expression in thermal burns of human skin for determination of wound age. In: Medicine, Science and the Law. 2004 ; Vol. 44, No. 3. pp. 222-226.
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