A substitution in the transmembrane region of the glycoprotein leads to an unstable attenuation of machupo virus

Michael Patterson, Takaaki Koma, Alexey Seregin, Cheng Huang, Milagros Miller, Jennifer Smith, Nadezhda Yun, Jeanon Smith, Slobodan Paessler

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Machupo virus (MACV) is the etiologic agent of Bolivian hemorrhagic fever (BHF). Utilizing a reverse-genetics system recently developed, we report the rescue of a rationally modified recombinant MACV containing a single mutation in the transmembrane region of the glycoprotein. Following challenge of susceptible mice, we identified a significant reduction in virulence in the novel virus. We also identified an instability leading to reversion of the single mutation to a wild-type genotype.

Original languageEnglish (US)
Pages (from-to)10995-10999
Number of pages5
JournalJournal of Virology
Volume88
Issue number18
DOIs
StatePublished - 2014

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Machupo virus
New World Arenaviruses
American Hemorrhagic Fever
glycoproteins
Glycoproteins
mutation
Reverse Genetics
Mutation
fever
Virulence
virulence
Genotype
Viruses
viruses
genotype
mice

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Medicine(all)

Cite this

A substitution in the transmembrane region of the glycoprotein leads to an unstable attenuation of machupo virus. / Patterson, Michael; Koma, Takaaki; Seregin, Alexey; Huang, Cheng; Miller, Milagros; Smith, Jennifer; Yun, Nadezhda; Smith, Jeanon; Paessler, Slobodan.

In: Journal of Virology, Vol. 88, No. 18, 2014, p. 10995-10999.

Research output: Contribution to journalArticle

Patterson, Michael ; Koma, Takaaki ; Seregin, Alexey ; Huang, Cheng ; Miller, Milagros ; Smith, Jennifer ; Yun, Nadezhda ; Smith, Jeanon ; Paessler, Slobodan. / A substitution in the transmembrane region of the glycoprotein leads to an unstable attenuation of machupo virus. In: Journal of Virology. 2014 ; Vol. 88, No. 18. pp. 10995-10999.
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