A substitution in the transmembrane region of the glycoprotein leads to an unstable attenuation of machupo virus

Michael Patterson; Takaaki Koma; Alexey Seregin; Cheng Huang; Milagros Miller; Jennifer Smith; Nadezhda E Yun; Jeanon Smith; Slobodan Paessler

doi:10.1128/JVI.01007-14

A substitution in the transmembrane region of the glycoprotein leads to an unstable attenuation of machupo virus

Michael Patterson, Takaaki Koma, Alexey Seregin, Cheng Huang, Milagros Miller, Jennifer Smith, Nadezhda E Yun, Jeanon Smith, Slobodan Paessler

Pathology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Machupo virus (MACV) is the etiologic agent of Bolivian hemorrhagic fever (BHF). Utilizing a reverse-genetics system recently developed, we report the rescue of a rationally modified recombinant MACV containing a single mutation in the transmembrane region of the glycoprotein. Following challenge of susceptible mice, we identified a significant reduction in virulence in the novel virus. We also identified an instability leading to reversion of the single mutation to a wild-type genotype.

Original language	English (US)
Pages (from-to)	10995-10999
Number of pages	5
Journal	Journal of virology
Volume	88
Issue number	18
DOIs	https://doi.org/10.1128/JVI.01007-14
State	Published - 2014

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

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10.1128/JVI.01007-14

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abstract = "Machupo virus (MACV) is the etiologic agent of Bolivian hemorrhagic fever (BHF). Utilizing a reverse-genetics system recently developed, we report the rescue of a rationally modified recombinant MACV containing a single mutation in the transmembrane region of the glycoprotein. Following challenge of susceptible mice, we identified a significant reduction in virulence in the novel virus. We also identified an instability leading to reversion of the single mutation to a wild-type genotype.",

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AU - Patterson, Michael

AU - Koma, Takaaki

AU - Seregin, Alexey

AU - Huang, Cheng

AU - Miller, Milagros

AU - Smith, Jennifer

AU - Yun, Nadezhda E

AU - Smith, Jeanon

AU - Paessler, Slobodan

PY - 2014

Y1 - 2014

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AB - Machupo virus (MACV) is the etiologic agent of Bolivian hemorrhagic fever (BHF). Utilizing a reverse-genetics system recently developed, we report the rescue of a rationally modified recombinant MACV containing a single mutation in the transmembrane region of the glycoprotein. Following challenge of susceptible mice, we identified a significant reduction in virulence in the novel virus. We also identified an instability leading to reversion of the single mutation to a wild-type genotype.

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EP - 10999

JO - Journal of virology

JF - Journal of virology

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A substitution in the transmembrane region of the glycoprotein leads to an unstable attenuation of machupo virus

Abstract

ASJC Scopus subject areas

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