A substitution in the transmembrane region of the glycoprotein leads to an unstable attenuation of machupo virus

Michael Patterson, Takaaki Koma, Alexey Seregin, Cheng Huang, Milagros Miller, Jennifer Smith, Nadezhda Yun, Jeanon Smith, Slobodan Paessler

Research output: Contribution to journalArticle

8 Scopus citations


Machupo virus (MACV) is the etiologic agent of Bolivian hemorrhagic fever (BHF). Utilizing a reverse-genetics system recently developed, we report the rescue of a rationally modified recombinant MACV containing a single mutation in the transmembrane region of the glycoprotein. Following challenge of susceptible mice, we identified a significant reduction in virulence in the novel virus. We also identified an instability leading to reversion of the single mutation to a wild-type genotype.

Original languageEnglish (US)
Pages (from-to)10995-10999
Number of pages5
JournalJournal of virology
Issue number18
StatePublished - 2014


ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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