A subunit of the mammalian oligosaccharyltransferase, DAD1, interacts with Mcl-1, one of the bcl-2 protein family

Tomoko Makishima, M. Yoshimi, S. Komiyama, N. Hara, T. Nishimoto

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

DAD1 is a mammalian homologue of Saccharomyces cerevisiae Ost2p, a subunit of the oligosaccharyltransferase complex. Loss of its function induces apoptosis in hamster BHK21 cells. By means of a two-hybrid method involving DAD1 as bait, the C-terminal region of Mcl-1, one of the bcl-2 family, was isolated. Consistently, DAD1 binds well to Mcl-1 in COS cells when overexpressed. On deletion analysis, the C-terminal domain of Mcl-1 containing BH2 (bcl-2 homologous domain) was found to be essential for the interaction with DAD1. On the other hand, the C-terminal half of DAD1 was concluded to be essential for the interaction with Mcl-1. Surprisingly, a ΔC-DAD1 mutant lacking only 4 amino acid residues from the C-terminus did not complement the tsBN7 mutation, while it interacted well with Mcl-1. In contrast, ΔN-DAD1 lacking 20 amino acid residues from the N-terminus still exhibited the ability to complement the tsBN7 mutation. Thus, the C-terminus of DAD1 was suggested to play an important role in N-linked glycosylation and to complement the tsBN7 mutation. Mcl-1 may be required for the inhibition of apoptotic cell death caused by a loss of DAD1.

Original languageEnglish (US)
Pages (from-to)399-405
Number of pages7
JournalJournal of Biochemistry
Volume128
Issue number3
StatePublished - 2000
Externally publishedYes

Fingerprint

Glycosylation
Amino Acids
Mutation
Cell death
Yeast
Two-Hybrid System Techniques
Proteins
COS Cells
Apoptosis
Cricetinae
Saccharomyces cerevisiae
Cell Death
dolichyl-diphosphooligosaccharide - protein glycotransferase

Keywords

  • Apoptosis
  • DAD1
  • Mcl-1
  • N-linked glycosylation
  • TsBN7

ASJC Scopus subject areas

  • Biochemistry

Cite this

A subunit of the mammalian oligosaccharyltransferase, DAD1, interacts with Mcl-1, one of the bcl-2 protein family. / Makishima, Tomoko; Yoshimi, M.; Komiyama, S.; Hara, N.; Nishimoto, T.

In: Journal of Biochemistry, Vol. 128, No. 3, 2000, p. 399-405.

Research output: Contribution to journalArticle

Makishima, Tomoko ; Yoshimi, M. ; Komiyama, S. ; Hara, N. ; Nishimoto, T. / A subunit of the mammalian oligosaccharyltransferase, DAD1, interacts with Mcl-1, one of the bcl-2 protein family. In: Journal of Biochemistry. 2000 ; Vol. 128, No. 3. pp. 399-405.
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