A thermal injury-induced circulating factor(s) compromises intestinal cell morphology, proliferation, and migration

Maryam Varedi, George H. Greeley, David Herndon, Ella Englander

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The effects of a 60% body surface area thermal injury in rats on the morphology and proliferation of the epithelium of the small intestine and the in vitro effects of serum collected from scalded rats on intestinal epithelial cells were investigated. Scald injury caused significant reductions in duodenal villus width and crypt dimensions, villus enterocytes changed in shape from columnar to cuboidal, and the number of goblet cells decreased. The proportion of bromodeoxyuridine- labeled S phase cells in crypts was also diminished. In vitro, incubation of intestinal epithelial cells (IEC-6) with scalded rat serum (SRS) collected at either 12 or 24 h after injury caused a disruption in the integrity of the confluent culture and induced the appearance of large denuded areas. SRS also decreased DNA synthesis and delayed wound closure in an in vitro wound-healing model. The thermal injury-induced changes in intestinal mucosal morphology and epithelial cell growth characteristics described in this study may underlie, in part, the mechanism(s) involved in the diminished absorption of nutrients, increased intestinal permeability, and sepsis in patients with thermal injury.

Original languageEnglish
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume277
Issue number1 40-1
StatePublished - Jul 1999

Fingerprint

Cell Movement
Hot Temperature
Cell Proliferation
Wounds and Injuries
Epithelial Cells
Serum
Goblet Cells
Enterocytes
Body Surface Area
Bromodeoxyuridine
S Phase
Wound Healing
Small Intestine
Permeability
Sepsis
Epithelium
Food
DNA
Growth
In Vitro Techniques

Keywords

  • Burn
  • Epithelium

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology
  • Physiology (medical)

Cite this

A thermal injury-induced circulating factor(s) compromises intestinal cell morphology, proliferation, and migration. / Varedi, Maryam; Greeley, George H.; Herndon, David; Englander, Ella.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 277, No. 1 40-1, 07.1999.

Research output: Contribution to journalArticle

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