A transcannula method for subcortical HRP gel implants: Inferior olive afferents in the rat

Susan M. Carlton; George R. Leichnetz; Eric G. Young; David J. Mayer

doi:10.1016/0361-9230(82)90084-3

A transcannula method for subcortical HRP gel implants: Inferior olive afferents in the rat

Susan M. Carlton, George R. Leichnetz, Eric G. Young, David J. Mayer

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

CARLTON, S. M., G. R. LEICHENTZ, E. G. YOUNG AND D. J. MAYER. A transcannula method for subcortical HRP gel implants: Inferior olive afferents in the rat. BRAIN RES. BULL. 8(6) 581-585, 1982.-A technique for using horseradish peroxidase in a polyacrylamide gel form for subcortical implants is described. In order to prevent uptake along the injection tract, the solid HRP gel pellet is delivered through a pre-implanted cannula to the target site. The appreciable advantages of using the HRP-transcannula technique are discussed in conjunction with a description of the afferents to the inferior olivary complex in the rat. The principal sources of afferents were the ipsilateral prerubral parafascicular nucleus, contralateral lateral cerebellar nucleus and dorsal column nuclei.

Original language	English (US)
Pages (from-to)	581-585
Number of pages	5
Journal	Brain Research Bulletin
Volume	8
Issue number	6
DOIs	https://doi.org/10.1016/0361-9230(82)90084-3
State	Published - Jun 1982

Keywords

Cannula implant
Horseradish peroxidase
Inferior olive
Polyacrylamide gel
Rat

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/0361-9230(82)90084-3

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@article{6f42a48678f849708e138e60fd985e9b,

title = "A transcannula method for subcortical HRP gel implants: Inferior olive afferents in the rat",

abstract = "CARLTON, S. M., G. R. LEICHENTZ, E. G. YOUNG AND D. J. MAYER. A transcannula method for subcortical HRP gel implants: Inferior olive afferents in the rat. BRAIN RES. BULL. 8(6) 581-585, 1982.-A technique for using horseradish peroxidase in a polyacrylamide gel form for subcortical implants is described. In order to prevent uptake along the injection tract, the solid HRP gel pellet is delivered through a pre-implanted cannula to the target site. The appreciable advantages of using the HRP-transcannula technique are discussed in conjunction with a description of the afferents to the inferior olivary complex in the rat. The principal sources of afferents were the ipsilateral prerubral parafascicular nucleus, contralateral lateral cerebellar nucleus and dorsal column nuclei.",

keywords = "Cannula implant, Horseradish peroxidase, Inferior olive, Polyacrylamide gel, Rat",

author = "Carlton, {Susan M.} and Leichnetz, {George R.} and Young, {Eric G.} and Mayer, {David J.}",

note = "Funding Information: The authorsw ould like to thank Mrs. Jo Anne Botkin for her excellentt echnicala ssistance.T his work was supportedi n part by NSF Grant BNS 782297t1o G. R. L. andP HS GrantD A-08576to D. J. M.",

year = "1982",

month = jun,

doi = "10.1016/0361-9230(82)90084-3",

language = "English (US)",

volume = "8",

pages = "581--585",

journal = "Journal of Electrophysiological Techniques",

issn = "0361-9230",

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T2 - Inferior olive afferents in the rat

AU - Carlton, Susan M.

AU - Leichnetz, George R.

AU - Young, Eric G.

AU - Mayer, David J.

N1 - Funding Information: The authorsw ould like to thank Mrs. Jo Anne Botkin for her excellentt echnicala ssistance.T his work was supportedi n part by NSF Grant BNS 782297t1o G. R. L. andP HS GrantD A-08576to D. J. M.

PY - 1982/6

Y1 - 1982/6

N2 - CARLTON, S. M., G. R. LEICHENTZ, E. G. YOUNG AND D. J. MAYER. A transcannula method for subcortical HRP gel implants: Inferior olive afferents in the rat. BRAIN RES. BULL. 8(6) 581-585, 1982.-A technique for using horseradish peroxidase in a polyacrylamide gel form for subcortical implants is described. In order to prevent uptake along the injection tract, the solid HRP gel pellet is delivered through a pre-implanted cannula to the target site. The appreciable advantages of using the HRP-transcannula technique are discussed in conjunction with a description of the afferents to the inferior olivary complex in the rat. The principal sources of afferents were the ipsilateral prerubral parafascicular nucleus, contralateral lateral cerebellar nucleus and dorsal column nuclei.

AB - CARLTON, S. M., G. R. LEICHENTZ, E. G. YOUNG AND D. J. MAYER. A transcannula method for subcortical HRP gel implants: Inferior olive afferents in the rat. BRAIN RES. BULL. 8(6) 581-585, 1982.-A technique for using horseradish peroxidase in a polyacrylamide gel form for subcortical implants is described. In order to prevent uptake along the injection tract, the solid HRP gel pellet is delivered through a pre-implanted cannula to the target site. The appreciable advantages of using the HRP-transcannula technique are discussed in conjunction with a description of the afferents to the inferior olivary complex in the rat. The principal sources of afferents were the ipsilateral prerubral parafascicular nucleus, contralateral lateral cerebellar nucleus and dorsal column nuclei.

KW - Cannula implant

KW - Horseradish peroxidase

KW - Inferior olive

KW - Polyacrylamide gel

KW - Rat

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ER -

A transcannula method for subcortical HRP gel implants: Inferior olive afferents in the rat

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