A two-codon mutant of cholera toxin lacking ADP-ribosylating activity functions as an effective adjuvant for eliciting mucosal and systemic cellular immune responses to peptide antigens

Dakshayani Lomada; Ratish Gambhira; Pramod N. Nehete; Faisal A. Guhad; Ashok K. Chopra; Johnny Peterson; K. Jagannadha Sastry

doi:10.1016/j.vaccine.2004.05.039

A two-codon mutant of cholera toxin lacking ADP-ribosylating activity functions as an effective adjuvant for eliciting mucosal and systemic cellular immune responses to peptide antigens

Dakshayani Lomada, Ratish Gambhira, Pramod N. Nehete, Faisal A. Guhad, Ashok K. Chopra, Johnny Peterson, K. Jagannadha Sastry

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Vaccination with peptide antigens is an effective strategy against mucosal viral infections. We tested a two-codon mutant of cholera toxin (CT-2*) lacking ADP-ribosylating activity and toxicity as a mucosal adjuvant for T cell epitope peptides for intranasal immunization of mice. Efficient induction of helper and cytotoxic T lymphocyte responses associated with TH1 cytokine production were observed in the systemic and mucosal compartments including nasal, gut, and vaginal associated lymphoid tissues. Single or multiple dosing with the peptide antigen and CT-2* induced strong memory immunity without tolerance. These results demonstrate CT-2* as a suitable mucosal adjuvant for priming antigen-specific cellular immune responses.

Original language	English (US)
Pages (from-to)	555-565
Number of pages	11
Journal	Vaccine
Volume	23
Issue number	4
DOIs	https://doi.org/10.1016/j.vaccine.2004.05.039
State	Published - Dec 9 2004
Externally published	Yes

Keywords

Cellular immune responses
Intranasal immunization
Mucosal tissues
Native and mutant cholera toxin

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/j.vaccine.2004.05.039

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A two-codon mutant of cholera toxin lacking ADP-ribosylating activity functions as an effective adjuvant for eliciting mucosal and systemic cellular immune responses to peptide antigens. / Lomada, Dakshayani; Gambhira, Ratish; Nehete, Pramod N. et al.
In: Vaccine, Vol. 23, No. 4, 09.12.2004, p. 555-565.

Research output: Contribution to journal › Article › peer-review

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title = "A two-codon mutant of cholera toxin lacking ADP-ribosylating activity functions as an effective adjuvant for eliciting mucosal and systemic cellular immune responses to peptide antigens",

abstract = "Vaccination with peptide antigens is an effective strategy against mucosal viral infections. We tested a two-codon mutant of cholera toxin (CT-2*) lacking ADP-ribosylating activity and toxicity as a mucosal adjuvant for T cell epitope peptides for intranasal immunization of mice. Efficient induction of helper and cytotoxic T lymphocyte responses associated with TH1 cytokine production were observed in the systemic and mucosal compartments including nasal, gut, and vaginal associated lymphoid tissues. Single or multiple dosing with the peptide antigen and CT-2* induced strong memory immunity without tolerance. These results demonstrate CT-2* as a suitable mucosal adjuvant for priming antigen-specific cellular immune responses.",

keywords = "Cellular immune responses, Intranasal immunization, Mucosal tissues, Native and mutant cholera toxin",

author = "Dakshayani Lomada and Ratish Gambhira and Nehete, {Pramod N.} and Guhad, {Faisal A.} and Chopra, {Ashok K.} and Johnny Peterson and Sastry, {K. Jagannadha}",

note = "Funding Information: This work was supported in part by National Institute of Allergy and Infectious Diseases grant AI 42694 to Sastry KJ and NIH grants—R01AI/DK 50086 and R01 AI 41611 to Chopra AK. All culture media were produced by the Central Media Lab, and all the synthetic peptides were prepared in the Synthetic Antigen Core Facility, both supported by funds from National Institute of Health Grant, CA 16672. We would also like to thank Mardelle Susman for editorial assistance and Pallavi Raja Manuri and Johny Simmons for FACS analysis.",

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doi = "10.1016/j.vaccine.2004.05.039",

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AU - Lomada, Dakshayani

AU - Gambhira, Ratish

AU - Nehete, Pramod N.

AU - Guhad, Faisal A.

AU - Chopra, Ashok K.

AU - Peterson, Johnny

AU - Sastry, K. Jagannadha

N1 - Funding Information: This work was supported in part by National Institute of Allergy and Infectious Diseases grant AI 42694 to Sastry KJ and NIH grants—R01AI/DK 50086 and R01 AI 41611 to Chopra AK. All culture media were produced by the Central Media Lab, and all the synthetic peptides were prepared in the Synthetic Antigen Core Facility, both supported by funds from National Institute of Health Grant, CA 16672. We would also like to thank Mardelle Susman for editorial assistance and Pallavi Raja Manuri and Johny Simmons for FACS analysis.

PY - 2004/12/9

Y1 - 2004/12/9

N2 - Vaccination with peptide antigens is an effective strategy against mucosal viral infections. We tested a two-codon mutant of cholera toxin (CT-2*) lacking ADP-ribosylating activity and toxicity as a mucosal adjuvant for T cell epitope peptides for intranasal immunization of mice. Efficient induction of helper and cytotoxic T lymphocyte responses associated with TH1 cytokine production were observed in the systemic and mucosal compartments including nasal, gut, and vaginal associated lymphoid tissues. Single or multiple dosing with the peptide antigen and CT-2* induced strong memory immunity without tolerance. These results demonstrate CT-2* as a suitable mucosal adjuvant for priming antigen-specific cellular immune responses.

AB - Vaccination with peptide antigens is an effective strategy against mucosal viral infections. We tested a two-codon mutant of cholera toxin (CT-2*) lacking ADP-ribosylating activity and toxicity as a mucosal adjuvant for T cell epitope peptides for intranasal immunization of mice. Efficient induction of helper and cytotoxic T lymphocyte responses associated with TH1 cytokine production were observed in the systemic and mucosal compartments including nasal, gut, and vaginal associated lymphoid tissues. Single or multiple dosing with the peptide antigen and CT-2* induced strong memory immunity without tolerance. These results demonstrate CT-2* as a suitable mucosal adjuvant for priming antigen-specific cellular immune responses.

KW - Cellular immune responses

KW - Intranasal immunization

KW - Mucosal tissues

KW - Native and mutant cholera toxin

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A two-codon mutant of cholera toxin lacking ADP-ribosylating activity functions as an effective adjuvant for eliciting mucosal and systemic cellular immune responses to peptide antigens

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ASJC Scopus subject areas

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