A two-codon mutant of cholera toxin lacking ADP-ribosylating activity functions as an effective adjuvant for eliciting mucosal and systemic cellular immune responses to peptide antigens

Dakshayani Lomada, Ratish Gambhira, Pramod N. Nehete, Faisal A. Guhad, Ashok Chopra, Johnny Peterson, K. Jagannadha Sastry

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Vaccination with peptide antigens is an effective strategy against mucosal viral infections. We tested a two-codon mutant of cholera toxin (CT-2*) lacking ADP-ribosylating activity and toxicity as a mucosal adjuvant for T cell epitope peptides for intranasal immunization of mice. Efficient induction of helper and cytotoxic T lymphocyte responses associated with TH1 cytokine production were observed in the systemic and mucosal compartments including nasal, gut, and vaginal associated lymphoid tissues. Single or multiple dosing with the peptide antigen and CT-2* induced strong memory immunity without tolerance. These results demonstrate CT-2* as a suitable mucosal adjuvant for priming antigen-specific cellular immune responses.

Original languageEnglish (US)
Pages (from-to)555-565
Number of pages11
JournalVaccine
Volume23
Issue number4
DOIs
StatePublished - Dec 9 2004

Fingerprint

cholera toxin
Cholera Toxin
codons
Cellular Immunity
Codon
cell-mediated immunity
Adenosine Diphosphate
adjuvants
peptides
antigens
Antigens
mutants
Peptides
cytotoxic T-lymphocytes
T-Lymphocyte Epitopes
Cytotoxic T-Lymphocytes
Lymphoid Tissue
Virus Diseases
Helper-Inducer T-Lymphocytes
Nose

Keywords

  • Cellular immune responses
  • Intranasal immunization
  • Mucosal tissues
  • Native and mutant cholera toxin

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

A two-codon mutant of cholera toxin lacking ADP-ribosylating activity functions as an effective adjuvant for eliciting mucosal and systemic cellular immune responses to peptide antigens. / Lomada, Dakshayani; Gambhira, Ratish; Nehete, Pramod N.; Guhad, Faisal A.; Chopra, Ashok; Peterson, Johnny; Sastry, K. Jagannadha.

In: Vaccine, Vol. 23, No. 4, 09.12.2004, p. 555-565.

Research output: Contribution to journalArticle

@article{5c6246616d1341ef8edfdc9e6dc8ba8a,
title = "A two-codon mutant of cholera toxin lacking ADP-ribosylating activity functions as an effective adjuvant for eliciting mucosal and systemic cellular immune responses to peptide antigens",
abstract = "Vaccination with peptide antigens is an effective strategy against mucosal viral infections. We tested a two-codon mutant of cholera toxin (CT-2*) lacking ADP-ribosylating activity and toxicity as a mucosal adjuvant for T cell epitope peptides for intranasal immunization of mice. Efficient induction of helper and cytotoxic T lymphocyte responses associated with TH1 cytokine production were observed in the systemic and mucosal compartments including nasal, gut, and vaginal associated lymphoid tissues. Single or multiple dosing with the peptide antigen and CT-2* induced strong memory immunity without tolerance. These results demonstrate CT-2* as a suitable mucosal adjuvant for priming antigen-specific cellular immune responses.",
keywords = "Cellular immune responses, Intranasal immunization, Mucosal tissues, Native and mutant cholera toxin",
author = "Dakshayani Lomada and Ratish Gambhira and Nehete, {Pramod N.} and Guhad, {Faisal A.} and Ashok Chopra and Johnny Peterson and Sastry, {K. Jagannadha}",
year = "2004",
month = "12",
day = "9",
doi = "10.1016/j.vaccine.2004.05.039",
language = "English (US)",
volume = "23",
pages = "555--565",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "4",

}

TY - JOUR

T1 - A two-codon mutant of cholera toxin lacking ADP-ribosylating activity functions as an effective adjuvant for eliciting mucosal and systemic cellular immune responses to peptide antigens

AU - Lomada, Dakshayani

AU - Gambhira, Ratish

AU - Nehete, Pramod N.

AU - Guhad, Faisal A.

AU - Chopra, Ashok

AU - Peterson, Johnny

AU - Sastry, K. Jagannadha

PY - 2004/12/9

Y1 - 2004/12/9

N2 - Vaccination with peptide antigens is an effective strategy against mucosal viral infections. We tested a two-codon mutant of cholera toxin (CT-2*) lacking ADP-ribosylating activity and toxicity as a mucosal adjuvant for T cell epitope peptides for intranasal immunization of mice. Efficient induction of helper and cytotoxic T lymphocyte responses associated with TH1 cytokine production were observed in the systemic and mucosal compartments including nasal, gut, and vaginal associated lymphoid tissues. Single or multiple dosing with the peptide antigen and CT-2* induced strong memory immunity without tolerance. These results demonstrate CT-2* as a suitable mucosal adjuvant for priming antigen-specific cellular immune responses.

AB - Vaccination with peptide antigens is an effective strategy against mucosal viral infections. We tested a two-codon mutant of cholera toxin (CT-2*) lacking ADP-ribosylating activity and toxicity as a mucosal adjuvant for T cell epitope peptides for intranasal immunization of mice. Efficient induction of helper and cytotoxic T lymphocyte responses associated with TH1 cytokine production were observed in the systemic and mucosal compartments including nasal, gut, and vaginal associated lymphoid tissues. Single or multiple dosing with the peptide antigen and CT-2* induced strong memory immunity without tolerance. These results demonstrate CT-2* as a suitable mucosal adjuvant for priming antigen-specific cellular immune responses.

KW - Cellular immune responses

KW - Intranasal immunization

KW - Mucosal tissues

KW - Native and mutant cholera toxin

UR - http://www.scopus.com/inward/record.url?scp=7744235981&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=7744235981&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2004.05.039

DO - 10.1016/j.vaccine.2004.05.039

M3 - Article

VL - 23

SP - 555

EP - 565

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 4

ER -