A two-codon mutant of cholera toxin lacking ADP-ribosylating activity functions as an effective adjuvant for eliciting mucosal and systemic cellular immune responses to peptide antigens

Dakshayani Lomada, Ratish Gambhira, Pramod N. Nehete, Faisal A. Guhad, Ashok Chopra, Johnny Peterson, K. Jagannadha Sastry

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9 Scopus citations

Abstract

Vaccination with peptide antigens is an effective strategy against mucosal viral infections. We tested a two-codon mutant of cholera toxin (CT-2*) lacking ADP-ribosylating activity and toxicity as a mucosal adjuvant for T cell epitope peptides for intranasal immunization of mice. Efficient induction of helper and cytotoxic T lymphocyte responses associated with TH1 cytokine production were observed in the systemic and mucosal compartments including nasal, gut, and vaginal associated lymphoid tissues. Single or multiple dosing with the peptide antigen and CT-2* induced strong memory immunity without tolerance. These results demonstrate CT-2* as a suitable mucosal adjuvant for priming antigen-specific cellular immune responses.

Original languageEnglish (US)
Pages (from-to)555-565
Number of pages11
JournalVaccine
Volume23
Issue number4
DOIs
StatePublished - Dec 9 2004

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Keywords

  • Cellular immune responses
  • Intranasal immunization
  • Mucosal tissues
  • Native and mutant cholera toxin

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

A two-codon mutant of cholera toxin lacking ADP-ribosylating activity functions as an effective adjuvant for eliciting mucosal and systemic cellular immune responses to peptide antigens. / Lomada, Dakshayani; Gambhira, Ratish; Nehete, Pramod N.; Guhad, Faisal A.; Chopra, Ashok; Peterson, Johnny; Sastry, K. Jagannadha.

In: Vaccine, Vol. 23, No. 4, 09.12.2004, p. 555-565.

Research output: Contribution to journalArticle