A unique and simple approach to improve sensitivity in 15N-NMR relaxation measurements for NH3+ groups

Application to a protein-DNA complex

Dan Nguyen, Ganesh L.R. Lokesh, David E. Volk, Junji Iwahara

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

NMR spectroscopy is a powerful tool for research on protein dynamics. In the past decade, there has been significant progress in the development of NMR methods for studying charged side chains. In particular, NMR methods for lysine side-chain NH3+ groups have been proven to be powerful for investigating the dynamics of hydrogen bonds or ion pairs that play important roles in biological processes. However, relatively low sensitivity has been a major practical issue in NMR experiments on NH3+ groups. In this paper, we present a unique and simple approach to improve sensitivity in 15N relaxation measurements for NH3+ groups. In this approach, the efficiency of coherence transfers for the desired components are maximized, whereas undesired anti-phase or multi-spin order components are purged through pulse schemes and rapid relaxation. For lysine side-chain NH3+ groups of a protein-DNA complex, we compared the data obtained with the previous and new pulse sequences under the same conditions and confirmed that the 15N relaxation parameters were consistent for these datasets. While retaining accuracy in measuring 15N relaxation, our new pulse sequences for NH3+ groups allowed an 82% increase in detection sensitivity of 15N longitudinal and transverse relaxation measurements.

Original languageEnglish (US)
Article number1355
JournalMolecules
Volume22
Issue number8
DOIs
StatePublished - Aug 1 2017

Fingerprint

Lysine
deoxyribonucleic acid
Nuclear magnetic resonance
proteins
Biological Phenomena
nuclear magnetic resonance
sensitivity
DNA
lysine
Hydrogen
Proteins
Magnetic Resonance Spectroscopy
Ions
Nuclear magnetic resonance spectroscopy
pulses
Hydrogen bonds
Research
hydrogen ions
retaining
hydrogen bonds

Keywords

  • Dynamics
  • Ion pairs
  • NH groups
  • NMR relaxation
  • Protein side chains

ASJC Scopus subject areas

  • Medicine(all)
  • Organic Chemistry

Cite this

A unique and simple approach to improve sensitivity in 15N-NMR relaxation measurements for NH3+ groups : Application to a protein-DNA complex. / Nguyen, Dan; Lokesh, Ganesh L.R.; Volk, David E.; Iwahara, Junji.

In: Molecules, Vol. 22, No. 8, 1355, 01.08.2017.

Research output: Contribution to journalArticle

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