A unique antiphospholipid assay recognizing phospholipid mixture compared with criteria antiphospholipid immunoassays in lupus patients

Y. Zuo, R. Willis, E. Papalardo, M. Petri, E. N. Harris, A. Schleh, K. Deceulaer, M. Smikle, L. M. Vilá, J. D. Reveille, G. S. Alarcón, Emilio Gonzalez

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Background While essential for the classification of antiphospholipid syndrome (APS), anticardiolipin (aCL) assays lack specificity and anti-β2glycoproteinI (anti-β2GPI) assays lack sensitivity in this regard. Our aim was to perform a comparative analysis of the APhL ELISA assay (IgG/IgM) and criteria antiphospholipid (aPL) immunoassays in identifying APS-related clinical manifestations in a large group of patients with systemic lupus erythematosus (SLE). Methods Serum samples from 1178 patients from the Hopkins (n = 543), LUMINA (n = 588) and Jamaican SLE cohorts (n = 47) were examined for IgG/IgM positivity in aCL (in-house), anti-β2GPI (two commercial kits) and APhL (Louisville APL) ELISA assays. Correlation of assay positivity with clinical manifestations and sensitivity, specificity, positive and negative predictive values and likelihood ratios were evaluated. A case series analysis was also performed in patients for whom there was isolated positivity in the specific aPL assays. Results The prevalence of aCL positivity was 34.9%, anti-β2GPI kit A was 22.6%, APhL was 11.5% and anti-β2GPI kit B was 7.6% in the study population. Anti-β2GPI kit B, aCL and APhL assays were correlated with venous thrombosis, while only APhL was significantly correlated with arterial thrombosis and consistently correlated with pregnancy-related morbidity. No significant correlations were noted for anti-β2GPI kit A. Sensitivity was greatest for aCL assays followed by anti-β2GPI kit A, APhL and anti-β2GPI kit B, while specificity was greatest and equal for anti-β2GPI kit B and APhL assays. Conclusions Overall, APhL antibodies, especially IgG, represent a promising biomarker for the classification of APS patients in the context of autoimmunity and in risk assessment with regards to pregnancy morbidity and thrombotic manifestations.

Original languageEnglish (US)
Pages (from-to)606-615
Number of pages10
JournalLupus
Volume26
Issue number6
DOIs
StatePublished - May 1 2017

Keywords

  • Anticardiolipin antibodies
  • antiphospholipid syndrome
  • classification
  • pregnancy morbidity
  • thrombosis

ASJC Scopus subject areas

  • Rheumatology

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    Zuo, Y., Willis, R., Papalardo, E., Petri, M., Harris, E. N., Schleh, A., Deceulaer, K., Smikle, M., Vilá, L. M., Reveille, J. D., Alarcón, G. S., & Gonzalez, E. (2017). A unique antiphospholipid assay recognizing phospholipid mixture compared with criteria antiphospholipid immunoassays in lupus patients. Lupus, 26(6), 606-615. https://doi.org/10.1177/0961203316671812