A Wolbachia symbiont in Aedes aegypti disrupts mosquito egg development to a greater extent when mosquitoes feed on nonhuman versus human blood

Conor J. McMeniman, Grant L. Hughes, Scott L. O'Neill

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

A vertebrate bloodmeal is required by female mosquitoes of most species to obtain nutrients for egg maturation. The yellowfever mosquito, Aedes aegypti (L.), feeds predominantly on humans, despite having the capacity to use blood from other hosts for this process. Here, we report that female Ae. aegypti infected with a virulent strain of the intracellular bacterium Wolbachia pipientis (wMelPop) from Drosophila melanogaster (Meigen) have a reduced ability to use blood for egg development. Blood feeding by wMelPop-infected females on mouse, guinea pig, or chicken hosts resulted in a near complete abolishment of reproductive output associated with both a decline in the numbers of eggs oviposited as well as the hatching rate of successfully laid eggs. In contrast, the reproductive output of wMelPop-infected females fed human blood was only mildly affected in comparison to individuals fed animal blood sources. Blood-feeding assays over two reproductive cycles definitively illustrated a nutritional interaction between host blood source and egg development in wMelPop-infected Ae. aegypti. Removal of Wolbachia from mosquitoes using antibiotic treatment rescued egg development on all blood sources. Further investigation of this phenotype may provide new insights into the nutritional basis of mosquito anthropophily.

Original languageEnglish (US)
Pages (from-to)76-84
Number of pages9
JournalJournal of Medical Entomology
Volume48
Issue number1
DOIs
StatePublished - Jan 2011
Externally publishedYes

Fingerprint

Wolbachia
Aedes
Aedes aegypti
Culicidae
symbionts
Ovum
blood
Eggs
reproductive performance
Wolbachia pipientis
Drosophila melanogaster
guinea pigs
Vertebrates
Chickens
Guinea Pigs
hatching
antibiotics
vertebrates
chickens
Anti-Bacterial Agents

Keywords

  • anthropophily
  • mosquito
  • reproduction
  • Wolbachia

ASJC Scopus subject areas

  • Insect Science
  • veterinary(all)
  • Infectious Diseases
  • Parasitology

Cite this

A Wolbachia symbiont in Aedes aegypti disrupts mosquito egg development to a greater extent when mosquitoes feed on nonhuman versus human blood. / McMeniman, Conor J.; Hughes, Grant L.; O'Neill, Scott L.

In: Journal of Medical Entomology, Vol. 48, No. 1, 01.2011, p. 76-84.

Research output: Contribution to journalArticle

@article{9cd7254ce57f416e8381dadb177f1117,
title = "A Wolbachia symbiont in Aedes aegypti disrupts mosquito egg development to a greater extent when mosquitoes feed on nonhuman versus human blood",
abstract = "A vertebrate bloodmeal is required by female mosquitoes of most species to obtain nutrients for egg maturation. The yellowfever mosquito, Aedes aegypti (L.), feeds predominantly on humans, despite having the capacity to use blood from other hosts for this process. Here, we report that female Ae. aegypti infected with a virulent strain of the intracellular bacterium Wolbachia pipientis (wMelPop) from Drosophila melanogaster (Meigen) have a reduced ability to use blood for egg development. Blood feeding by wMelPop-infected females on mouse, guinea pig, or chicken hosts resulted in a near complete abolishment of reproductive output associated with both a decline in the numbers of eggs oviposited as well as the hatching rate of successfully laid eggs. In contrast, the reproductive output of wMelPop-infected females fed human blood was only mildly affected in comparison to individuals fed animal blood sources. Blood-feeding assays over two reproductive cycles definitively illustrated a nutritional interaction between host blood source and egg development in wMelPop-infected Ae. aegypti. Removal of Wolbachia from mosquitoes using antibiotic treatment rescued egg development on all blood sources. Further investigation of this phenotype may provide new insights into the nutritional basis of mosquito anthropophily.",
keywords = "anthropophily, mosquito, reproduction, Wolbachia",
author = "McMeniman, {Conor J.} and Hughes, {Grant L.} and O'Neill, {Scott L.}",
year = "2011",
month = "1",
doi = "10.1603/ME09188",
language = "English (US)",
volume = "48",
pages = "76--84",
journal = "Journal of Medical Entomology",
issn = "0022-2585",
publisher = "Entomological Society of America",
number = "1",

}

TY - JOUR

T1 - A Wolbachia symbiont in Aedes aegypti disrupts mosquito egg development to a greater extent when mosquitoes feed on nonhuman versus human blood

AU - McMeniman, Conor J.

AU - Hughes, Grant L.

AU - O'Neill, Scott L.

PY - 2011/1

Y1 - 2011/1

N2 - A vertebrate bloodmeal is required by female mosquitoes of most species to obtain nutrients for egg maturation. The yellowfever mosquito, Aedes aegypti (L.), feeds predominantly on humans, despite having the capacity to use blood from other hosts for this process. Here, we report that female Ae. aegypti infected with a virulent strain of the intracellular bacterium Wolbachia pipientis (wMelPop) from Drosophila melanogaster (Meigen) have a reduced ability to use blood for egg development. Blood feeding by wMelPop-infected females on mouse, guinea pig, or chicken hosts resulted in a near complete abolishment of reproductive output associated with both a decline in the numbers of eggs oviposited as well as the hatching rate of successfully laid eggs. In contrast, the reproductive output of wMelPop-infected females fed human blood was only mildly affected in comparison to individuals fed animal blood sources. Blood-feeding assays over two reproductive cycles definitively illustrated a nutritional interaction between host blood source and egg development in wMelPop-infected Ae. aegypti. Removal of Wolbachia from mosquitoes using antibiotic treatment rescued egg development on all blood sources. Further investigation of this phenotype may provide new insights into the nutritional basis of mosquito anthropophily.

AB - A vertebrate bloodmeal is required by female mosquitoes of most species to obtain nutrients for egg maturation. The yellowfever mosquito, Aedes aegypti (L.), feeds predominantly on humans, despite having the capacity to use blood from other hosts for this process. Here, we report that female Ae. aegypti infected with a virulent strain of the intracellular bacterium Wolbachia pipientis (wMelPop) from Drosophila melanogaster (Meigen) have a reduced ability to use blood for egg development. Blood feeding by wMelPop-infected females on mouse, guinea pig, or chicken hosts resulted in a near complete abolishment of reproductive output associated with both a decline in the numbers of eggs oviposited as well as the hatching rate of successfully laid eggs. In contrast, the reproductive output of wMelPop-infected females fed human blood was only mildly affected in comparison to individuals fed animal blood sources. Blood-feeding assays over two reproductive cycles definitively illustrated a nutritional interaction between host blood source and egg development in wMelPop-infected Ae. aegypti. Removal of Wolbachia from mosquitoes using antibiotic treatment rescued egg development on all blood sources. Further investigation of this phenotype may provide new insights into the nutritional basis of mosquito anthropophily.

KW - anthropophily

KW - mosquito

KW - reproduction

KW - Wolbachia

UR - http://www.scopus.com/inward/record.url?scp=79551620846&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79551620846&partnerID=8YFLogxK

U2 - 10.1603/ME09188

DO - 10.1603/ME09188

M3 - Article

VL - 48

SP - 76

EP - 84

JO - Journal of Medical Entomology

JF - Journal of Medical Entomology

SN - 0022-2585

IS - 1

ER -