A Yersinia pestis lpxM-mutant live vaccine induces enhanced immunity against bubonic plague in mice and guinea pigs

V. A. Feodorova; L. N. Pan'kina; E. P. Savostina; L. V. Sayapina; V. L. Motin; S. V. Dentovskaya; R. Z. Shaikhutdinova; S. A. Ivanov; B. Lindner; A. N. Kondakova; O. V. Bystrova; N. A. Kocharova; S. N. Senchenkova; O. Holst; G. B. Pier; Y. A. Knirel; A. P. Anisimov

doi:10.1016/j.vaccine.2007.08.055

A Yersinia pestis lpxM-mutant live vaccine induces enhanced immunity against bubonic plague in mice and guinea pigs

V. A. Feodorova, L. N. Pan'kina, E. P. Savostina, L. V. Sayapina, V. L. Motin, S. V. Dentovskaya, R. Z. Shaikhutdinova, S. A. Ivanov, B. Lindner, A. N. Kondakova, O. V. Bystrova, N. A. Kocharova, S. N. Senchenkova, O. Holst, G. B. Pier, Y. A. Knirel, A. P. Anisimov

Pathology

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

The lpxM mutant of the live vaccine Yersinia pestis EV NIIEG strain synthesising a less toxic penta-acylated lipopolysaccharide was found to be avirulent in mice and guinea pigs, notably showing no measurable virulence in Balb/c mice which do retain some susceptibility to the parental strain itself. Twenty-one days after a single injection of the lpxM-mutant, 85-100% protection was achieved in outbred mice and guinea pigs, whereas a 43% protection rate was achieved in Balb/c mice given single low doses (10³ to 2.5 × 10⁴ CFU) of this vaccine. A subcutaneous challenge with 2000 median lethal doses (equal to 20,000 CFU) of fully virulent Y. pestis 231 strain, is a 6-10-fold higher dose than that which the EV NIIEG itself can protect against.

Original language	English (US)
Pages (from-to)	7620-7628
Number of pages	9
Journal	Vaccine
Volume	25
Issue number	44
DOIs	https://doi.org/10.1016/j.vaccine.2007.08.055
State	Published - Nov 1 2007

Keywords

Live plague vaccine
Yersinia pestis EV NIIEG
lpxM(msbB) mutant

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/j.vaccine.2007.08.055

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Feodorova, V. A., Pan'kina, L. N., Savostina, E. P., Sayapina, L. V., Motin, V. L., Dentovskaya, S. V., Shaikhutdinova, R. Z., Ivanov, S. A., Lindner, B., Kondakova, A. N., Bystrova, O. V., Kocharova, N. A., Senchenkova, S. N., Holst, O., Pier, G. B., Knirel, Y. A., & Anisimov, A. P. (2007). A Yersinia pestis lpxM-mutant live vaccine induces enhanced immunity against bubonic plague in mice and guinea pigs. Vaccine, 25(44), 7620-7628. https://doi.org/10.1016/j.vaccine.2007.08.055

Feodorova, VA, Pan'kina, LN, Savostina, EP, Sayapina, LV, Motin, VL, Dentovskaya, SV, Shaikhutdinova, RZ, Ivanov, SA, Lindner, B, Kondakova, AN, Bystrova, OV, Kocharova, NA, Senchenkova, SN, Holst, O, Pier, GB, Knirel, YA & Anisimov, AP 2007, 'A Yersinia pestis lpxM-mutant live vaccine induces enhanced immunity against bubonic plague in mice and guinea pigs', Vaccine, vol. 25, no. 44, pp. 7620-7628. https://doi.org/10.1016/j.vaccine.2007.08.055

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title = "A Yersinia pestis lpxM-mutant live vaccine induces enhanced immunity against bubonic plague in mice and guinea pigs",

abstract = "The lpxM mutant of the live vaccine Yersinia pestis EV NIIEG strain synthesising a less toxic penta-acylated lipopolysaccharide was found to be avirulent in mice and guinea pigs, notably showing no measurable virulence in Balb/c mice which do retain some susceptibility to the parental strain itself. Twenty-one days after a single injection of the lpxM-mutant, 85-100% protection was achieved in outbred mice and guinea pigs, whereas a 43% protection rate was achieved in Balb/c mice given single low doses (103 to 2.5 × 104 CFU) of this vaccine. A subcutaneous challenge with 2000 median lethal doses (equal to 20,000 CFU) of fully virulent Y. pestis 231 strain, is a 6-10-fold higher dose than that which the EV NIIEG itself can protect against.",

keywords = "Live plague vaccine, Yersinia pestis EV NIIEG, lpxM(msbB) mutant",

author = "Feodorova, {V. A.} and Pan'kina, {L. N.} and Savostina, {E. P.} and Sayapina, {L. V.} and Motin, {V. L.} and Dentovskaya, {S. V.} and Shaikhutdinova, {R. Z.} and Ivanov, {S. A.} and B. Lindner and Kondakova, {A. N.} and Bystrova, {O. V.} and Kocharova, {N. A.} and Senchenkova, {S. N.} and O. Holst and Pier, {G. B.} and Knirel, {Y. A.} and Anisimov, {A. P.}",

note = "Funding Information: This work was supported by the Russian Foundation for Basic Research (grants 06-04-49280 and 03-04-48067), the Western RCE, NIH (award 1-U54-AI-057156), the Council on Grants at the President of the Russian Federation for Support of Young Russian Scientists (grant MK-5304.2007.4), the German Research Foundation (grant LI-448-1), the US National Institutes of Health (grant AI-46706-06) and the International Science and Technology Center (ISTC) (grant 3730).",

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AU - Feodorova, V. A.

AU - Pan'kina, L. N.

AU - Savostina, E. P.

AU - Sayapina, L. V.

AU - Motin, V. L.

AU - Dentovskaya, S. V.

AU - Shaikhutdinova, R. Z.

AU - Ivanov, S. A.

AU - Lindner, B.

AU - Kondakova, A. N.

AU - Bystrova, O. V.

AU - Kocharova, N. A.

AU - Senchenkova, S. N.

AU - Holst, O.

AU - Pier, G. B.

AU - Knirel, Y. A.

AU - Anisimov, A. P.

N1 - Funding Information: This work was supported by the Russian Foundation for Basic Research (grants 06-04-49280 and 03-04-48067), the Western RCE, NIH (award 1-U54-AI-057156), the Council on Grants at the President of the Russian Federation for Support of Young Russian Scientists (grant MK-5304.2007.4), the German Research Foundation (grant LI-448-1), the US National Institutes of Health (grant AI-46706-06) and the International Science and Technology Center (ISTC) (grant 3730).

PY - 2007/11/1

Y1 - 2007/11/1

N2 - The lpxM mutant of the live vaccine Yersinia pestis EV NIIEG strain synthesising a less toxic penta-acylated lipopolysaccharide was found to be avirulent in mice and guinea pigs, notably showing no measurable virulence in Balb/c mice which do retain some susceptibility to the parental strain itself. Twenty-one days after a single injection of the lpxM-mutant, 85-100% protection was achieved in outbred mice and guinea pigs, whereas a 43% protection rate was achieved in Balb/c mice given single low doses (103 to 2.5 × 104 CFU) of this vaccine. A subcutaneous challenge with 2000 median lethal doses (equal to 20,000 CFU) of fully virulent Y. pestis 231 strain, is a 6-10-fold higher dose than that which the EV NIIEG itself can protect against.

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A Yersinia pestis lpxM-mutant live vaccine induces enhanced immunity against bubonic plague in mice and guinea pigs

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Keywords

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