A Yersinia pestis lpxM-mutant live vaccine induces enhanced immunity against bubonic plague in mice and guinea pigs

V. A. Feodorova, L. N. Pan'kina, E. P. Savostina, L. V. Sayapina, V. L. Motin, S. V. Dentovskaya, R. Z. Shaikhutdinova, S. A. Ivanov, B. Lindner, A. N. Kondakova, O. V. Bystrova, N. A. Kocharova, S. N. Senchenkova, O. Holst, G. B. Pier, Y. A. Knirel, A. P. Anisimov

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31 Scopus citations

Abstract

The lpxM mutant of the live vaccine Yersinia pestis EV NIIEG strain synthesising a less toxic penta-acylated lipopolysaccharide was found to be avirulent in mice and guinea pigs, notably showing no measurable virulence in Balb/c mice which do retain some susceptibility to the parental strain itself. Twenty-one days after a single injection of the lpxM-mutant, 85-100% protection was achieved in outbred mice and guinea pigs, whereas a 43% protection rate was achieved in Balb/c mice given single low doses (103 to 2.5 × 104 CFU) of this vaccine. A subcutaneous challenge with 2000 median lethal doses (equal to 20,000 CFU) of fully virulent Y. pestis 231 strain, is a 6-10-fold higher dose than that which the EV NIIEG itself can protect against.

Original languageEnglish (US)
Pages (from-to)7620-7628
Number of pages9
JournalVaccine
Volume25
Issue number44
DOIs
StatePublished - Nov 1 2007

Keywords

  • Live plague vaccine
  • Yersinia pestis EV NIIEG
  • lpxM(msbB) mutant

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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    Feodorova, V. A., Pan'kina, L. N., Savostina, E. P., Sayapina, L. V., Motin, V. L., Dentovskaya, S. V., Shaikhutdinova, R. Z., Ivanov, S. A., Lindner, B., Kondakova, A. N., Bystrova, O. V., Kocharova, N. A., Senchenkova, S. N., Holst, O., Pier, G. B., Knirel, Y. A., & Anisimov, A. P. (2007). A Yersinia pestis lpxM-mutant live vaccine induces enhanced immunity against bubonic plague in mice and guinea pigs. Vaccine, 25(44), 7620-7628. https://doi.org/10.1016/j.vaccine.2007.08.055