A Zika virus vaccine expressing premembrane-envelope-NS1 polyprotein

Anzhong Li, Jingyou Yu, Mijia Lu, Yuanmei Ma, Zayed Attia, Chao Shan, Miaoge Xue, Xueya Liang, Kelsey Craig, Nirajkumar Makadiya, Jennifer J. He, Ryan Jennings, Pei-Yong Shi, Mark E. Peeples, Shan Lu Liu, Prosper N. Boyaka, Jianrong Li

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Current efforts to develop Zika virus (ZIKV) subunit vaccines have been focused on pre-membrane (prM) and envelope (E) proteins, but the role of NS1 in ZIKV-specific immune response and protection is poorly understood. Here, we develop an attenuated recombinant vesicular stomatitis virus (rVSV)-based vaccine expressing ZIKV prM-E-NS1 as a polyprotein. This vectored vaccine candidate is attenuated in mice, where a single immunization induces ZIKV-specific antibody and T cell immune responses that provide protection against ZIKV challenge. Co-expression of prM, E, and NS1 induces significantly higher levels of Th2 and Th17 cytokine responses than prM-E. In addition, NS1 alone is capable of conferring partial protection against ZIKV infection in mice even though it does not induce neutralizing antibodies. These results demonstrate that attenuated rVSV co-expressing prM, E, and NS1 is a promising vaccine candidate for protection against ZIKV infection and highlights an important role for NS1 in ZIKV-specific cellular immune responses.

Original languageEnglish (US)
Article number3067
JournalNature Communications
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2018

Fingerprint

vaccines
Polyproteins
viruses
Viruses
envelopes
Vaccines
Membranes
Vesicular Stomatitis
membranes
infectious diseases
Subunit Vaccines
antibodies
mice
Neutralizing Antibodies
Cellular Immunity
Zika Virus
Immunization
T-cells
Cytokines
T-Lymphocytes

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Li, A., Yu, J., Lu, M., Ma, Y., Attia, Z., Shan, C., ... Li, J. (2018). A Zika virus vaccine expressing premembrane-envelope-NS1 polyprotein. Nature Communications, 9(1), [3067]. https://doi.org/10.1038/s41467-018-05276-4

A Zika virus vaccine expressing premembrane-envelope-NS1 polyprotein. / Li, Anzhong; Yu, Jingyou; Lu, Mijia; Ma, Yuanmei; Attia, Zayed; Shan, Chao; Xue, Miaoge; Liang, Xueya; Craig, Kelsey; Makadiya, Nirajkumar; He, Jennifer J.; Jennings, Ryan; Shi, Pei-Yong; Peeples, Mark E.; Liu, Shan Lu; Boyaka, Prosper N.; Li, Jianrong.

In: Nature Communications, Vol. 9, No. 1, 3067, 01.12.2018.

Research output: Contribution to journalArticle

Li, A, Yu, J, Lu, M, Ma, Y, Attia, Z, Shan, C, Xue, M, Liang, X, Craig, K, Makadiya, N, He, JJ, Jennings, R, Shi, P-Y, Peeples, ME, Liu, SL, Boyaka, PN & Li, J 2018, 'A Zika virus vaccine expressing premembrane-envelope-NS1 polyprotein', Nature Communications, vol. 9, no. 1, 3067. https://doi.org/10.1038/s41467-018-05276-4
Li, Anzhong ; Yu, Jingyou ; Lu, Mijia ; Ma, Yuanmei ; Attia, Zayed ; Shan, Chao ; Xue, Miaoge ; Liang, Xueya ; Craig, Kelsey ; Makadiya, Nirajkumar ; He, Jennifer J. ; Jennings, Ryan ; Shi, Pei-Yong ; Peeples, Mark E. ; Liu, Shan Lu ; Boyaka, Prosper N. ; Li, Jianrong. / A Zika virus vaccine expressing premembrane-envelope-NS1 polyprotein. In: Nature Communications. 2018 ; Vol. 9, No. 1.
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