Absence of degenerative changes in retinal and uveal capillary pericytes in diabetic rats

Ronald Tilton, L. S. LaRose, C. Kilo, J. R. Williamson

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Ultrastructural morphometric techniques were used to quantify pericyte degeneration in retinal and uveal capillaries of streptozotocin-diabetic rats in order to assess the suitability of this small rodent model of diabetes for studies of the pathogenesis of microvascular eye disease in diabetic humans. Male, Sprague-Dawley rats were killed by intraaortic perfusion of fixative 6 and 9 mos after induction of diabetes with 50 mg/kg streptozotocin. No differences were evident between diabetics and age-matched controls in capillary circumference, numbers of endothelial cells per capillary, and capillary cytoplasmic area of retinal, choroidal, and iridial vessels. Capillary basement membrane width and the percentage of the capillary circumference covered by pericytes were increased in retinas of diabetic vs age-matched control rats after 9 mos of diabetes (P < 0.05), but no differences were evident in the number of pericyte processes per capillary and the percentage of vessels with pericyte nuclei. No differences in pericyte distributions were observed between control and diabetic rats in the choriocapillaris and iris after 9 mos of diabetes. These findings indicate that retinal capillary basement membrane thickening precedes any evidence of pericyte degenerative changes and suggest that pericyte degeneration analogous to that associated with human diabetic microangiopathy does not occur in this experimental animal model.

Original languageEnglish (US)
Pages (from-to)716-721
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume27
Issue number5
StatePublished - 1986
Externally publishedYes

Fingerprint

Pericytes
Streptozocin
Basement Membrane
Diabetic Angiopathies
Fixatives
Retinal Degeneration
Eye Diseases
Iris
Sprague Dawley Rats
Retina
Rodentia
Endothelial Cells
Animal Models
Perfusion

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Absence of degenerative changes in retinal and uveal capillary pericytes in diabetic rats. / Tilton, Ronald; LaRose, L. S.; Kilo, C.; Williamson, J. R.

In: Investigative Ophthalmology and Visual Science, Vol. 27, No. 5, 1986, p. 716-721.

Research output: Contribution to journalArticle

Tilton, Ronald ; LaRose, L. S. ; Kilo, C. ; Williamson, J. R. / Absence of degenerative changes in retinal and uveal capillary pericytes in diabetic rats. In: Investigative Ophthalmology and Visual Science. 1986 ; Vol. 27, No. 5. pp. 716-721.
@article{0708fdcd8c754678bd0f5c4b08ef202e,
title = "Absence of degenerative changes in retinal and uveal capillary pericytes in diabetic rats",
abstract = "Ultrastructural morphometric techniques were used to quantify pericyte degeneration in retinal and uveal capillaries of streptozotocin-diabetic rats in order to assess the suitability of this small rodent model of diabetes for studies of the pathogenesis of microvascular eye disease in diabetic humans. Male, Sprague-Dawley rats were killed by intraaortic perfusion of fixative 6 and 9 mos after induction of diabetes with 50 mg/kg streptozotocin. No differences were evident between diabetics and age-matched controls in capillary circumference, numbers of endothelial cells per capillary, and capillary cytoplasmic area of retinal, choroidal, and iridial vessels. Capillary basement membrane width and the percentage of the capillary circumference covered by pericytes were increased in retinas of diabetic vs age-matched control rats after 9 mos of diabetes (P < 0.05), but no differences were evident in the number of pericyte processes per capillary and the percentage of vessels with pericyte nuclei. No differences in pericyte distributions were observed between control and diabetic rats in the choriocapillaris and iris after 9 mos of diabetes. These findings indicate that retinal capillary basement membrane thickening precedes any evidence of pericyte degenerative changes and suggest that pericyte degeneration analogous to that associated with human diabetic microangiopathy does not occur in this experimental animal model.",
author = "Ronald Tilton and LaRose, {L. S.} and C. Kilo and Williamson, {J. R.}",
year = "1986",
language = "English (US)",
volume = "27",
pages = "716--721",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "5",

}

TY - JOUR

T1 - Absence of degenerative changes in retinal and uveal capillary pericytes in diabetic rats

AU - Tilton, Ronald

AU - LaRose, L. S.

AU - Kilo, C.

AU - Williamson, J. R.

PY - 1986

Y1 - 1986

N2 - Ultrastructural morphometric techniques were used to quantify pericyte degeneration in retinal and uveal capillaries of streptozotocin-diabetic rats in order to assess the suitability of this small rodent model of diabetes for studies of the pathogenesis of microvascular eye disease in diabetic humans. Male, Sprague-Dawley rats were killed by intraaortic perfusion of fixative 6 and 9 mos after induction of diabetes with 50 mg/kg streptozotocin. No differences were evident between diabetics and age-matched controls in capillary circumference, numbers of endothelial cells per capillary, and capillary cytoplasmic area of retinal, choroidal, and iridial vessels. Capillary basement membrane width and the percentage of the capillary circumference covered by pericytes were increased in retinas of diabetic vs age-matched control rats after 9 mos of diabetes (P < 0.05), but no differences were evident in the number of pericyte processes per capillary and the percentage of vessels with pericyte nuclei. No differences in pericyte distributions were observed between control and diabetic rats in the choriocapillaris and iris after 9 mos of diabetes. These findings indicate that retinal capillary basement membrane thickening precedes any evidence of pericyte degenerative changes and suggest that pericyte degeneration analogous to that associated with human diabetic microangiopathy does not occur in this experimental animal model.

AB - Ultrastructural morphometric techniques were used to quantify pericyte degeneration in retinal and uveal capillaries of streptozotocin-diabetic rats in order to assess the suitability of this small rodent model of diabetes for studies of the pathogenesis of microvascular eye disease in diabetic humans. Male, Sprague-Dawley rats were killed by intraaortic perfusion of fixative 6 and 9 mos after induction of diabetes with 50 mg/kg streptozotocin. No differences were evident between diabetics and age-matched controls in capillary circumference, numbers of endothelial cells per capillary, and capillary cytoplasmic area of retinal, choroidal, and iridial vessels. Capillary basement membrane width and the percentage of the capillary circumference covered by pericytes were increased in retinas of diabetic vs age-matched control rats after 9 mos of diabetes (P < 0.05), but no differences were evident in the number of pericyte processes per capillary and the percentage of vessels with pericyte nuclei. No differences in pericyte distributions were observed between control and diabetic rats in the choriocapillaris and iris after 9 mos of diabetes. These findings indicate that retinal capillary basement membrane thickening precedes any evidence of pericyte degenerative changes and suggest that pericyte degeneration analogous to that associated with human diabetic microangiopathy does not occur in this experimental animal model.

UR - http://www.scopus.com/inward/record.url?scp=0022481004&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022481004&partnerID=8YFLogxK

M3 - Article

C2 - 3700020

AN - SCOPUS:0022481004

VL - 27

SP - 716

EP - 721

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 5

ER -