Absence of demonstrable linkage of human genes for enzymes of the purine and pyrimidine salvage pathways in human-mouse somatic cell hybrids

C. Long, T. Chan, V. Levytska, T. Kusano, H. Green

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A number of different reduced human-mouse hybrids have been analyzed for the presence of human enzymes of the purine and pyrimidine salvage pathways. Homologous mouse and human enzymes were characterized by isoelectric fractionation or gel electrophoresis, and the species of origin of the enzyme in hybrid clones was determined. Hybrids selected for one of the human enzymes, thymidine kinase, adenine phosphoribosyltransferase, or hypoxanthine phosphoribosyltransferase, were each found to contain the selected enzyme but not the other two. Neither human adenosine kinase nor human deoxycytidine (cytidine) deaminase was present in any of the hybrid clones. A human 5′-nucleotidase was present in two hybrid clones containing human hypoxanthine phosphoribosyltransferase, but the genes for the two enzymes are not linked. The genes for the purine and pyrimidine salvage enzymes appear to be dispersed in the human genome.

Original languageEnglish (US)
Pages (from-to)283-297
Number of pages15
JournalBiochemical Genetics
Volume9
Issue number3
DOIs
StatePublished - Jul 1973
Externally publishedYes

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Salvaging
Hybrid Cells
pyrimidines
purines
somatic cells
linkage (genetics)
Genes
enzyme
gene
mice
Enzymes
enzymes
genes
Hypoxanthine Phosphoribosyltransferase
clone
deoxycytidine deaminase
Clone Cells
hypoxanthine
clones
Adenine Phosphoribosyltransferase

ASJC Scopus subject areas

  • Genetics
  • Biochemistry

Cite this

Absence of demonstrable linkage of human genes for enzymes of the purine and pyrimidine salvage pathways in human-mouse somatic cell hybrids. / Long, C.; Chan, T.; Levytska, V.; Kusano, T.; Green, H.

In: Biochemical Genetics, Vol. 9, No. 3, 07.1973, p. 283-297.

Research output: Contribution to journalArticle

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