Absence of formation of benzo[a]pyrene/DNA adducts in the cuttlefish (Sepia officinalis, mollusca: cephalopoda)

P. G. Lee, Leejane Lu, J. J. Salazar, V. Holoubek

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Benzo[a]pyrene (B[a]P] injected intramuscularly into the base of the arms of cuttlefish was released continuously from the injection site and removed from the organism. Only a portion of the compound accumulated in the body. Twenty-four hr after its injection, 75% of B[a]P applied in olive oil was removed from the cuttlefish, and 1.2% was found in the body outside the head, the site of injection. If the carcinogen was dissolved in dimethylformamide, the removal of B[a]P was slower, so that only 18% of the injected B[a]P was removed from the organism and 0.36% accumulated in the body outside the head 24 hr after injection. The high level of B[a]P in gills and hemolymph 4 hr after injection and the kinetics of the decrease of its concentration with time indicate that these two organs could be involved in the excretion of B[a]P from the body. The B[a]P/DNA adducts characteristic for vertebrates could not be demonstrated in gills, skin, brain, hepatopancreas, and lymphocytes of the cuttlefish 24 hr after injection of B[a]P. The dose of the carcinogen injected into the cuttlefish was 2-4 times higher than the dose resulting in the formation of a high level of B[a]P/DNA adducts in the vertebrates. A different metabolism of B[a]P in the tissue of cephalopods, compared to vertebrates, could be less favorable to the process leading to malignant transformation and could explain the absence from the literature of reports of tumors in cephalopods.

Original languageEnglish (US)
Pages (from-to)70-73
Number of pages4
JournalEnvironmental and Molecular Mutagenesis
Volume23
Issue number1
StatePublished - 1994

Fingerprint

Sepia
Cephalopoda
Decapodiformes
Mollusca
Benzo(a)pyrene
pyrene
DNA
Injections
Vertebrates
vertebrate
Carcinogens
cephalopod
carcinogen
Head
Hepatopancreas
Dimethylformamide
Lymphocytes
Hemolymph
benzo(a)pyrene-DNA adduct
Metabolism

ASJC Scopus subject areas

  • Genetics
  • Environmental Science(all)
  • Environmental Chemistry
  • Health, Toxicology and Mutagenesis
  • Genetics(clinical)
  • Toxicology

Cite this

Absence of formation of benzo[a]pyrene/DNA adducts in the cuttlefish (Sepia officinalis, mollusca : cephalopoda). / Lee, P. G.; Lu, Leejane; Salazar, J. J.; Holoubek, V.

In: Environmental and Molecular Mutagenesis, Vol. 23, No. 1, 1994, p. 70-73.

Research output: Contribution to journalArticle

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