Abstract
Increased gastrointestinal absorption of intact antigen with systemic immunization has been considered a major etiologic factor in the development of food sensitivity. We attempted to test this hypothesis in infants with suspected food protein-induced enter colitis by measuring serum ovalbumin (OVA) concentrations after ingestion of egg white (prior to the performance of good challenges to establish this diagnosis). We first noted significant underestimation of serum OVA concentrations in the presence of even low serum anti-OVA antibody concentrations (>1:12). Next, using selected noninhibitory sera, we found that all infants studied absorbed some OVA, there was no correlation between serum OVA levels and age (3-11 months), and there was no significant difference between serum OVA concentrations in infants who subsequently had positive oral food challenge responses (120 ±67 ng/ml) and a matched group with negative challenges (102 ±80). These data do not support the hypothesis that "intestinal closure" (antigen exclusion) occurs in the neonatal period or the role of antigen absorption as the major etiological factor in the development of food sensitivity. Better methods of quantitating macromolecular absorption must be developed before the role of antigen absorption in food sensitivity can be assessed. Of note, urinary excretion of intact OVA also occurred. This varied greatly from one voiding to the next and continuing for at least 13 hr after ingestion.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 781-788 |
| Number of pages | 8 |
| Journal | Digestive Diseases and Sciences |
| Volume | 34 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 1989 |
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Keywords
- food protein-induced enterocolitis
- food sensitivity
- infants
- macromolecular absorption
- ovalbumin
ASJC Scopus subject areas
- Gastroenterology
Cite this
Absorption of food protein antigen in infants with food protein-induced enterocolitis. / Powell, Geraldine Keating; McDonald, Phillip J.; Van Sickle, Greggory J.; Goldblum, Randall M.
In: Digestive Diseases and Sciences, Vol. 34, No. 5, 05.1989, p. 781-788.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Absorption of food protein antigen in infants with food protein-induced enterocolitis
AU - Powell, Geraldine Keating
AU - McDonald, Phillip J.
AU - Van Sickle, Greggory J.
AU - Goldblum, Randall M.
PY - 1989/5
Y1 - 1989/5
N2 - Increased gastrointestinal absorption of intact antigen with systemic immunization has been considered a major etiologic factor in the development of food sensitivity. We attempted to test this hypothesis in infants with suspected food protein-induced enter colitis by measuring serum ovalbumin (OVA) concentrations after ingestion of egg white (prior to the performance of good challenges to establish this diagnosis). We first noted significant underestimation of serum OVA concentrations in the presence of even low serum anti-OVA antibody concentrations (>1:12). Next, using selected noninhibitory sera, we found that all infants studied absorbed some OVA, there was no correlation between serum OVA levels and age (3-11 months), and there was no significant difference between serum OVA concentrations in infants who subsequently had positive oral food challenge responses (120 ±67 ng/ml) and a matched group with negative challenges (102 ±80). These data do not support the hypothesis that "intestinal closure" (antigen exclusion) occurs in the neonatal period or the role of antigen absorption as the major etiological factor in the development of food sensitivity. Better methods of quantitating macromolecular absorption must be developed before the role of antigen absorption in food sensitivity can be assessed. Of note, urinary excretion of intact OVA also occurred. This varied greatly from one voiding to the next and continuing for at least 13 hr after ingestion.
AB - Increased gastrointestinal absorption of intact antigen with systemic immunization has been considered a major etiologic factor in the development of food sensitivity. We attempted to test this hypothesis in infants with suspected food protein-induced enter colitis by measuring serum ovalbumin (OVA) concentrations after ingestion of egg white (prior to the performance of good challenges to establish this diagnosis). We first noted significant underestimation of serum OVA concentrations in the presence of even low serum anti-OVA antibody concentrations (>1:12). Next, using selected noninhibitory sera, we found that all infants studied absorbed some OVA, there was no correlation between serum OVA levels and age (3-11 months), and there was no significant difference between serum OVA concentrations in infants who subsequently had positive oral food challenge responses (120 ±67 ng/ml) and a matched group with negative challenges (102 ±80). These data do not support the hypothesis that "intestinal closure" (antigen exclusion) occurs in the neonatal period or the role of antigen absorption as the major etiological factor in the development of food sensitivity. Better methods of quantitating macromolecular absorption must be developed before the role of antigen absorption in food sensitivity can be assessed. Of note, urinary excretion of intact OVA also occurred. This varied greatly from one voiding to the next and continuing for at least 13 hr after ingestion.
KW - food protein-induced enterocolitis
KW - food sensitivity
KW - infants
KW - macromolecular absorption
KW - ovalbumin
UR - http://www.scopus.com/inward/record.url?scp=0024410298&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024410298&partnerID=8YFLogxK
U2 - 10.1007/BF01540354
DO - 10.1007/BF01540354
M3 - Article
C2 - 2714153
AN - SCOPUS:0024410298
VL - 34
SP - 781
EP - 788
JO - Digestive Diseases and Sciences
JF - Digestive Diseases and Sciences
SN - 0163-2116
IS - 5
ER -