Acanthamoeba polyphaga mimivirus stability in environmental and clinical substrates

Implications for virus detection and isolation

Fábio P. Dornas, Lorena C.F. Silva, Gabriel M. De Almeida, Rafael Kroon Campos, Paulo V.M. Boratto, Ana P.M. Franco-Luiz, Bernard La Scola, Paulo C.P. Ferreira, Erna G. Kroon, Jônatas S. Abrahão

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Viruses are extremely diverse and abundant and are present in countless environments. Giant viruses of the Megavirales order have emerged as a fascinating research topic for virologists around the world. As evidence of their ubiquity and ecological impact, mimiviruses have been found in multiple environmental samples. However, isolation of these viruses from environmental samples is inefficient, mainly due to methodological limitations and lack of information regarding the interactions between viruses and substrates. In this work, we demonstrate the long-lasting stability of mimivirus in environmental (freshwater and saline water) and hospital (ventilator plastic device tube) substrates, showing the detection of infectious particles after more than 9 months. In addition, an enrichment protocol was implemented that remarkably increased mimivirus detection from all tested substrates, including field tests. Moreover, biological, morphological and genetic tests revealed that the enrichment protocol maintained mimivirus particle integrity. In conclusion, our work demonstrated the stability of APMV in samples of environmental and health interest and proposed a reliable and easy protocol to improve giant virus isolation. The data presented here can guide future giant virus detection and isolation studies. Copyright:

Original languageEnglish (US)
Article numbere87811
JournalPloS one
Volume9
Issue number2
DOIs
StatePublished - Feb 3 2014
Externally publishedYes

Fingerprint

Acanthamoeba polyphaga mimivirus
Mimiviridae
Mimivirus
Viruses
viruses
Substrates
Environmental Health
Mechanical Ventilators
Fresh Water
Plastics
ventilators
saline water
sampling
Equipment and Supplies
Giant Viruses
plastics
testing
Research
Health

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Dornas, F. P., Silva, L. C. F., De Almeida, G. M., Kroon Campos, R., Boratto, P. V. M., Franco-Luiz, A. P. M., ... Abrahão, J. S. (2014). Acanthamoeba polyphaga mimivirus stability in environmental and clinical substrates: Implications for virus detection and isolation. PloS one, 9(2), [e87811]. https://doi.org/10.1371/journal.pone.0087811

Acanthamoeba polyphaga mimivirus stability in environmental and clinical substrates : Implications for virus detection and isolation. / Dornas, Fábio P.; Silva, Lorena C.F.; De Almeida, Gabriel M.; Kroon Campos, Rafael; Boratto, Paulo V.M.; Franco-Luiz, Ana P.M.; La Scola, Bernard; Ferreira, Paulo C.P.; Kroon, Erna G.; Abrahão, Jônatas S.

In: PloS one, Vol. 9, No. 2, e87811, 03.02.2014.

Research output: Contribution to journalArticle

Dornas, FP, Silva, LCF, De Almeida, GM, Kroon Campos, R, Boratto, PVM, Franco-Luiz, APM, La Scola, B, Ferreira, PCP, Kroon, EG & Abrahão, JS 2014, 'Acanthamoeba polyphaga mimivirus stability in environmental and clinical substrates: Implications for virus detection and isolation', PloS one, vol. 9, no. 2, e87811. https://doi.org/10.1371/journal.pone.0087811
Dornas, Fábio P. ; Silva, Lorena C.F. ; De Almeida, Gabriel M. ; Kroon Campos, Rafael ; Boratto, Paulo V.M. ; Franco-Luiz, Ana P.M. ; La Scola, Bernard ; Ferreira, Paulo C.P. ; Kroon, Erna G. ; Abrahão, Jônatas S. / Acanthamoeba polyphaga mimivirus stability in environmental and clinical substrates : Implications for virus detection and isolation. In: PloS one. 2014 ; Vol. 9, No. 2.
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