Acceleration of R5 HIV replication by polymorphonuclear neutrophils in cultures of macrophages

Tsuyoshi Yoshida, Vickie C. Jones, Makiko Kobayashi, Xiao Dong Li, Richard B. Pollard, Fujio Suzuki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Cell-to-cell contact between monocyte-derived macrophages (MDM) and endothelial cells has resulted in the increased proliferation of CC chemokine receptor 5/M-tropic (R5) human immunodeficiency virus (HIV) in MDM. In the present study, R5 HIV replication was shown to be increased by polymorphonuclear neutrophils (PMN) in MDM cultures through the soluble factors released from these PMN. The replication of R5 HIV in MDM was greatly enhanced when PMN were added to cultures. An increase in the replication of R5 HIV was also demonstrated when the virus was replicated in MDM cultured in a double chamber transwell with PMN. Chemokine ligand (CCL) 2 and interleukin (IL)-10 were detected in culture fluids of PMN exposed to R5 HIV. The replication of R5 HIV was not accelerated in cultures of MDM and PMN in a double chamber transwell supplemented with a mixture of monoclonal antibodies directed against CCL2 and IL-10. Similarly, the replication of R5 HIV was accelerated in MDM cultures supplemented with a mixture of recombinant CCL2 and IL-10. These results indicated that, in response to the viral stimulation, PMN produce CCL2 and IL-10 and enhance the replication of R5 HIV in MDM cultures.

Original languageEnglish (US)
Pages (from-to)215-219
Number of pages5
JournalImmunology and Cell Biology
Volume85
Issue number3
DOIs
StatePublished - Apr 2007

Fingerprint

Macrophages
Virus Replication
Viruses
Cell culture
Neutrophils
HIV
Interleukin-10
CCR5 Receptors
Tropics
Endothelial cells
Chemokines
Endothelial Cells
Monoclonal Antibodies
Ligands
Fluids

Keywords

  • CCL2
  • HIV
  • Macrophages
  • Neutrophils

ASJC Scopus subject areas

  • Immunology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Acceleration of R5 HIV replication by polymorphonuclear neutrophils in cultures of macrophages. / Yoshida, Tsuyoshi; Jones, Vickie C.; Kobayashi, Makiko; Li, Xiao Dong; Pollard, Richard B.; Suzuki, Fujio.

In: Immunology and Cell Biology, Vol. 85, No. 3, 04.2007, p. 215-219.

Research output: Contribution to journalArticle

Yoshida, Tsuyoshi ; Jones, Vickie C. ; Kobayashi, Makiko ; Li, Xiao Dong ; Pollard, Richard B. ; Suzuki, Fujio. / Acceleration of R5 HIV replication by polymorphonuclear neutrophils in cultures of macrophages. In: Immunology and Cell Biology. 2007 ; Vol. 85, No. 3. pp. 215-219.
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