Accuracy, lesion bypass, strand displacement and translocation by DNA polymerases

Thomas A. Steitz, Yuhui Yin

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The structures of DNA polymerases from different families show common features and significant differences that shed light on the ability of these enzymes to accurately copy DNA and translocate. The structure of a B family DNA polymerase from phage RB69 exhibits an active-site closing conformational change in the fingers domain upon forming a ternary complex with primer template in deoxynucleoside triphosphate. The rotation of the fingers domain α-helices by 60° upon dNTP binding is analogous to the changes seen in other families of polymerases. When the 3′ terminus is bound to the editing 3′ exonuclease active site, the orientation of the DNA helix axis changes by 40° and the thumb domain re-orients with the DNA. Structures of substrate and product complexes of T7 RNA polymerase, a structural homologue of T7 DNA polymerase, show that family polymerases use the rotation conformational change of the fingers domain to translocate down the DNA. The fingers opening rotation that results in translocation is powered by the release of the product pyrophosphate and also enables the Pol I family polymerases to function as a helicase in displacing the downstream non-template strand from the template strand.

Original languageEnglish (US)
Pages (from-to)17-23
Number of pages7
JournalPhilosophical Transactions of the Royal Society B: Biological Sciences
Volume359
Issue number1441
DOIs
StatePublished - Jan 29 2004
Externally publishedYes

Fingerprint

DNA-directed DNA polymerase
bypass
DNA-Directed DNA Polymerase
lesion
translocation
Fingers
DNA
spleen exonuclease
active sites
Catalytic Domain
Bacteriophages
Thumb
pyrophosphates
DNA-directed RNA polymerase
bacteriophages
Substrates
Enzymes
enzymes
RNA
family

Keywords

  • DNA polymerase
  • Protein structure
  • Replication
  • RNA polymerase
  • Translocation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Accuracy, lesion bypass, strand displacement and translocation by DNA polymerases. / Steitz, Thomas A.; Yin, Yuhui.

In: Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 359, No. 1441, 29.01.2004, p. 17-23.

Research output: Contribution to journalArticle

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