TY - JOUR
T1 - Accuracy of thymine-thymine dimer bypass by Saccharomyces cerevisiae DNA polymerase η
AU - Washington, M. Todd
AU - Johnson, Robert E.
AU - Prakash, Satya
AU - Prakash, Louise
PY - 2000/3/28
Y1 - 2000/3/28
N2 - The Saccharomyces cerevisiae RAD30 gene functions in error-free replication of UV-damaged DNA. RAD30 encodes a DNA polymerase, Pol η, which inserts two adenines opposite the two thymines of a cis-syn thymine-thymine (T-T) dimer. Here we use steady-state kinetics to determine the accuracy of DNA synthesis opposite the T-T dimer. Surprisingly, the accuracy of DNA synthesis opposite the damaged DNA is nearly indistinguishable from that opposite non-damaged DNA, with frequencies of misincorporation of about 10- 2 to 10-3. These studies support the hypothesis that unlike most DNA polymerases, Pol η is able to tolerate distortions in DNA resulting from damage, which then enables the polymerase to utilize the intrinsic base pairing ability of the T-T dimer.
AB - The Saccharomyces cerevisiae RAD30 gene functions in error-free replication of UV-damaged DNA. RAD30 encodes a DNA polymerase, Pol η, which inserts two adenines opposite the two thymines of a cis-syn thymine-thymine (T-T) dimer. Here we use steady-state kinetics to determine the accuracy of DNA synthesis opposite the T-T dimer. Surprisingly, the accuracy of DNA synthesis opposite the damaged DNA is nearly indistinguishable from that opposite non-damaged DNA, with frequencies of misincorporation of about 10- 2 to 10-3. These studies support the hypothesis that unlike most DNA polymerases, Pol η is able to tolerate distortions in DNA resulting from damage, which then enables the polymerase to utilize the intrinsic base pairing ability of the T-T dimer.
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U2 - 10.1073/pnas.050491997
DO - 10.1073/pnas.050491997
M3 - Article
C2 - 10725365
AN - SCOPUS:0034724287
SN - 0027-8424
VL - 97
SP - 3094
EP - 3099
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 7
ER -