Accurate detection of enzymatic degradation processes of gelatin-alginate microcapsule by 1H NMR spectroscopy: Probing biodegradation mechanism and kinetics

Uyen Thi Do, Jiwon Kim, Quy Son Luu, Quynh Thi Nguyen, Taeho Jang, Yeeun Park, Hwicheol Shin, Nicholas Whiting, Dong-Ku Kang, Jas-Sung Kwon, Youngbok Lee

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

With an increase in the severity of environmental pollution caused by microbeads, the development of biodegradable microcapsules that can be applied in diverse fields has attracted significant attention. The degradation processes are directly related to biodegradable microcapsule creation with high stability and persistence. In this study, biodegradable microcapsules are synthesized via a complex coacervation approach using gelatin and alginate as the capsule main wall materials; additionally, enzyme-induced decomposition mechanisms are proposed by observing spectral changes in proton nuclear magnetic resonance (1H NMR) analyses. Additional analytical techniques confirm the chemical structure, morphology, and size distribution of the synthesized capsules; these uniform spherical microcapsules are 20-30 μm in size and possess a smooth surface. In addition to characterization, the microcapsules were exposed to targeted enzymes to investigate enzymatic effects using short-term and long-term degradation kinetics. Close inspection reveals that determination of the degradation rate constant of the major components in the capsule is feasible, and suggests two types of 4-stage degradation mechanisms that are enzyme-specific. These investigations demonstrate that capsule degradation can be explored in detail using 1H NMR spectroscopy to provide a viable strategy for monitoring degradation properties in the development of new biodegradable polymers.

Original languageEnglish (US)
Pages (from-to)120490
JournalCarbohydrate Polymers
Volume304
DOIs
StatePublished - Mar 15 2023
Externally publishedYes

Keywords

  • Capsules/chemistry
  • Gelatin
  • Alginates/chemistry
  • Kinetics
  • Magnetic Resonance Spectroscopy

Fingerprint

Dive into the research topics of 'Accurate detection of enzymatic degradation processes of gelatin-alginate microcapsule by 1H NMR spectroscopy: Probing biodegradation mechanism and kinetics'. Together they form a unique fingerprint.

Cite this