Accurate in vivo measurements of the corneal light backscattering in-depth distribution with optical coherence tomography for different hydration states

Alexander I. Kholodnykh, Kamran Hosseini, Kirill V. Larin, Alexandra A. Vassilieva, Rinat O. Esenaliev, Massoud Motamedi

Research output: Contribution to journalConference article

1 Scopus citations


Recently we reported measurements of the corneal in-depth light backscattering distribution (CDLBD) by optical coherence tomography (OCT) as a possible tool for investigation of the corneal hydration and estimation of water gradients inside the cornea. In this paper, we present additional results demonstrating a strong correlation (R = 0.99) between the amplitude of light backscattering as measured by OCT and the corneal thickness. In contrary to the well-known effect of the immediate increase observed in corneal opacity during corneal swelling, we observed an initial decrease of the amplitude of light backscatteriag as measured by OCT in the anterior part of the stroma during the swelling process. The possible explanation for this observation is discussed. Also methodological improvements for accurate measurements of the CDLBD in vivo are outlined.

Original languageEnglish (US)
Pages (from-to)12-15
Number of pages4
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - Oct 27 2004
EventProgress in Biomedical Optics and Imaging - Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VIII - San Jose, CA, United States
Duration: Jan 26 2004Jan 28 2004



  • Corneal backscattering
  • Corneal hydration control
  • Corneal thickness
  • Noninvasive monitoring
  • OCT

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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