Acetylation of H2AZ Lys 14 is associated with genome-wide gene activity in yeast

Catherine B. Millar, Feng Xu, Kangling Zhang, Michael Grunstein

Research output: Contribution to journalArticle

204 Citations (Scopus)

Abstract

Histone variants and their post-translational modifications help regulate chromosomal functions. Htz1 is an evolutionarily conserved H2A variant found at several promoters in the yeast Saccharomyces cerevisiae. In this study, we undertook a genome-wide analysis of Htz1 and its modifications in yeast. Using mass spectrometric analysis, we determined that Htz1 is acetylated at Lys 3, Lys 8, Lys 10, and Lys 14 within its N-terminal tail, with K14 being the most abundant acetylated site. ChIP and microarray analysis were then used to compare the location of Htz1-K14 acetylation to that of Htz1 genome-wide. The data presented here demonstrate that while Htz1 is associated preferentially with the promoters of repressed genes, K14 acetylation is enriched at the promoters of active genes, and requires two known histone acetyltransferases, Gcn5 and Esa1. In support of our genome-wide analysis, we found that the acetylatable lysines of Htz1 are required for its full deposition during nucleosome reassembly upon repression of PHO5. Since the majority of Htz1 acetylation is seen at active promoters, where nucleosomes are known to be disassembled, our data argue for a dynamic process in which reassembly of Htz1 is regulated by its acetylation at promoters during transcription.

Original languageEnglish (US)
Pages (from-to)711-722
Number of pages12
JournalGenes and Development
Volume20
Issue number6
DOIs
StatePublished - Mar 15 2006
Externally publishedYes

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Acetylation
Yeasts
Genome
Nucleosomes
Genes
Histone Acetyltransferases
Post Translational Protein Processing
Microarray Analysis
Histones
Lysine
Saccharomyces cerevisiae

Keywords

  • H2AZ
  • Histone acetylation
  • Histone variants
  • Htz1

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Acetylation of H2AZ Lys 14 is associated with genome-wide gene activity in yeast. / Millar, Catherine B.; Xu, Feng; Zhang, Kangling; Grunstein, Michael.

In: Genes and Development, Vol. 20, No. 6, 15.03.2006, p. 711-722.

Research output: Contribution to journalArticle

Millar, Catherine B. ; Xu, Feng ; Zhang, Kangling ; Grunstein, Michael. / Acetylation of H2AZ Lys 14 is associated with genome-wide gene activity in yeast. In: Genes and Development. 2006 ; Vol. 20, No. 6. pp. 711-722.
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