Acetylome analysis identifies SIRT1 targets in mRNA-processing and chromatin-remodeling in mouse liver

Sun Yee Kim, Choon Kiat Sim, Hui Tang, Weiping Han, Kangling Zhang, Feng Xu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Lysine acetylation is a post-translational modification found on numerous proteins, a strategy used in cell signaling to change protein activity in response to internal or external cues. Sirtuin 1 (SIRT1) is a central lysine deacetylase involved in a variety of cellular processes including metabolism, apoptosis, and DNA repair. Here we characterize the lysine acetylome in mouse liver, and by using a model of Sirt1-/-knockout mouse, show that SIRT1 regulates the deacetylation of 70 proteins in the liver in-vivo. Amongst these SIRT1-regulated proteins, we find that four RNA-processing proteins and a chromatin-remodeling protein can be deacetylated by SIRT1 directly in-vitro. The discovery that SIRT1 has a potential role in RNA-processing suggests a new layer of regulation in the variety of functions performed by SIRT1.

Original languageEnglish (US)
Article numbere0140619
JournalPLoS One
Volume10
Issue number10
DOIs
StatePublished - Oct 15 2015

Fingerprint

Sirtuin 1
Chromatin Assembly and Disassembly
Liver
Chromatin
chromatin
Messenger RNA
liver
mice
Processing
lysine
Lysine
Proteins
proteins
RNA
liver protein
Cell signaling
post-translational modification
acetylation
Acetylation
DNA repair

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Acetylome analysis identifies SIRT1 targets in mRNA-processing and chromatin-remodeling in mouse liver. / Kim, Sun Yee; Sim, Choon Kiat; Tang, Hui; Han, Weiping; Zhang, Kangling; Xu, Feng.

In: PLoS One, Vol. 10, No. 10, e0140619, 15.10.2015.

Research output: Contribution to journalArticle

Kim, Sun Yee ; Sim, Choon Kiat ; Tang, Hui ; Han, Weiping ; Zhang, Kangling ; Xu, Feng. / Acetylome analysis identifies SIRT1 targets in mRNA-processing and chromatin-remodeling in mouse liver. In: PLoS One. 2015 ; Vol. 10, No. 10.
@article{7895b2136e6a4e5692cd666d1ee2bd13,
title = "Acetylome analysis identifies SIRT1 targets in mRNA-processing and chromatin-remodeling in mouse liver",
abstract = "Lysine acetylation is a post-translational modification found on numerous proteins, a strategy used in cell signaling to change protein activity in response to internal or external cues. Sirtuin 1 (SIRT1) is a central lysine deacetylase involved in a variety of cellular processes including metabolism, apoptosis, and DNA repair. Here we characterize the lysine acetylome in mouse liver, and by using a model of Sirt1-/-knockout mouse, show that SIRT1 regulates the deacetylation of 70 proteins in the liver in-vivo. Amongst these SIRT1-regulated proteins, we find that four RNA-processing proteins and a chromatin-remodeling protein can be deacetylated by SIRT1 directly in-vitro. The discovery that SIRT1 has a potential role in RNA-processing suggests a new layer of regulation in the variety of functions performed by SIRT1.",
author = "Kim, {Sun Yee} and Sim, {Choon Kiat} and Hui Tang and Weiping Han and Kangling Zhang and Feng Xu",
year = "2015",
month = "10",
day = "15",
doi = "10.1371/journal.pone.0140619",
language = "English (US)",
volume = "10",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "10",

}

TY - JOUR

T1 - Acetylome analysis identifies SIRT1 targets in mRNA-processing and chromatin-remodeling in mouse liver

AU - Kim, Sun Yee

AU - Sim, Choon Kiat

AU - Tang, Hui

AU - Han, Weiping

AU - Zhang, Kangling

AU - Xu, Feng

PY - 2015/10/15

Y1 - 2015/10/15

N2 - Lysine acetylation is a post-translational modification found on numerous proteins, a strategy used in cell signaling to change protein activity in response to internal or external cues. Sirtuin 1 (SIRT1) is a central lysine deacetylase involved in a variety of cellular processes including metabolism, apoptosis, and DNA repair. Here we characterize the lysine acetylome in mouse liver, and by using a model of Sirt1-/-knockout mouse, show that SIRT1 regulates the deacetylation of 70 proteins in the liver in-vivo. Amongst these SIRT1-regulated proteins, we find that four RNA-processing proteins and a chromatin-remodeling protein can be deacetylated by SIRT1 directly in-vitro. The discovery that SIRT1 has a potential role in RNA-processing suggests a new layer of regulation in the variety of functions performed by SIRT1.

AB - Lysine acetylation is a post-translational modification found on numerous proteins, a strategy used in cell signaling to change protein activity in response to internal or external cues. Sirtuin 1 (SIRT1) is a central lysine deacetylase involved in a variety of cellular processes including metabolism, apoptosis, and DNA repair. Here we characterize the lysine acetylome in mouse liver, and by using a model of Sirt1-/-knockout mouse, show that SIRT1 regulates the deacetylation of 70 proteins in the liver in-vivo. Amongst these SIRT1-regulated proteins, we find that four RNA-processing proteins and a chromatin-remodeling protein can be deacetylated by SIRT1 directly in-vitro. The discovery that SIRT1 has a potential role in RNA-processing suggests a new layer of regulation in the variety of functions performed by SIRT1.

UR - http://www.scopus.com/inward/record.url?scp=84949033786&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84949033786&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0140619

DO - 10.1371/journal.pone.0140619

M3 - Article

VL - 10

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 10

M1 - e0140619

ER -