Acquisition of anoikis resistance up-regulates caveolin-1 expression in human non-small cell lung cancer cells

Hasseri Halim, Sudjit Luanpitpong, Pithi Chanvorachote

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Background: Anoikis is a key inhibitory step in the process of cancer cell metastasis. Knowledge regarding the adaptive response resulting in resistance to anoikis may benefit the development of new therapies. Materials and Methods: Anoikis-resistant cells were generated from anoikis-sensitive lung carcinoma cells and the underlying mechanism for this process was investigated. Results: Culturing H460 cells under suspended conditions caused spontaneous generation of anoikis-resistant H-AR1 and H-AR2 cells. We found that anoikis resistance in these cells caused caveolin-1 (CAV1) up-regulation. Using short hairpin RNA (shRNA), we confirmed that depletion of CAV1 rendered anoikis-resistant H-AR2 cells sensitive to anoikis. Furthermore, this study revealed that the acquisition of anoikis resistance induced CAV1 up-regulation through induction of CAV1 mRNA transcription. Conclusion: Our findings show CAV1 to be a key player in anoikis resistance and provide a novel mechanism regarding cancer cell adaptation, resulting in acquisition of anoikis resistance in lung cancer cells.

Original languageEnglish (US)
Pages (from-to)1649-1658
Number of pages10
JournalAnticancer Research
Volume32
Issue number5
StatePublished - May 1 2012

Keywords

  • Anoikis resistance
  • Cancer adaptation
  • Caveolin-1
  • H460 cells
  • Lung cancer
  • mRNA transcription

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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